Overview

  • Product name

  • Description

    Rabbit polyclonal to RTN3/HAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RTN3/HAP aa 550-600. The exact sequence is proprietary. (AAU81930.1).
    Database link: O95197

  • Positive control

  • General notes

     This product was previously labelled as RTN3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab225560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 113 kDa.

Target

  • Function

    May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
  • Tissue specificity

    Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
  • Sequence similarities

    Contains 1 reticulon domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASYIP antibody
    • Neuroendocrine specific protein like 2 antibody
    • Neuroendocrine-specific protein-like 2 antibody
    • Neuroendocrine-specific protein-like II antibody
    • NSP like protein II antibody
    • NSP-like protein 2 antibody
    • NSP-like protein II antibody
    • NSPL2 antibody
    • NSPLII antibody
    • Reticulon 3 antibody
    • Reticulon-3 antibody
    • Reticulon3 antibody
    • Rtn3 antibody
    • RTN3_HUMAN antibody
    see all

Images

  • All lanes : Anti-RTN3/HAP antibody (ab225560) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 113 kDa


    Exposure time: 30 seconds
  • RTN3/HAP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225560 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225560 at 0.4 µg/ml.

    Lane 1: ab225560 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab225560 has not yet been referenced specifically in any publications.

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