Overview

  • Product name

  • Description

    Rabbit polyclonal to RTN3/HAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human RTN3/HAP (internal sequence). The exact sequence is proprietary.
    Database link: O95197

  • Positive control

    • WB: HeLa whole cell and membrane extracts. IP: HeLa whole cell extract.
  • General notes

     This product was previously labelled as RTN3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IP 1/100 - 1/500.

Target

  • Function

    May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
  • Tissue specificity

    Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
  • Sequence similarities

    Contains 1 reticulon domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASYIP antibody
    • Neuroendocrine specific protein like 2 antibody
    • Neuroendocrine-specific protein-like 2 antibody
    • Neuroendocrine-specific protein-like II antibody
    • NSP like protein II antibody
    • NSP-like protein 2 antibody
    • NSP-like protein II antibody
    • NSPL2 antibody
    • NSPLII antibody
    • Reticulon 3 antibody
    • Reticulon-3 antibody
    • Reticulon3 antibody
    • Rtn3 antibody
    • RTN3_HUMAN antibody
    see all

Images

  • All lanes : Anti-RTN3/HAP antibody (ab226787) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) membrane extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 113 kDa



    5% SDS-PAGE gel.

  • RTN3/HAP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab226787. Western blot was performed from the immunoprecipitate using ab226787. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in HeLa whole cell extract.

    Lane 2: ab226787 IP in HeLa whole cell extract.

References

ab226787 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226787.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up