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    rtn3hap-antibody-ab246911.pdf

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Cell Biology Apoptosis Mitochondrial
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Anti-RTN3/HAP antibody (ab246911)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (ab246911)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)

Key features and details

  • Rabbit polyclonal to RTN3/HAP
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RTN3/HAP protein (ab153066)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RTN3/HAP antibody
    See all RTN3/HAP primary antibodies
  • Description

    Rabbit polyclonal to RTN3/HAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human RTN3/HAP aa 81-217.
    Sequence:

    IMTSSFLSSSEIHNTGLTILHGEKSHVLGSQPILAKEGKDHLDLLDMKKM EKPQGTSNNVSDSSVSLAAGVHCDRPSIPASFPEHPAFLSKKIGQVEEQI DKETKNPNGVSSREAKTALDADDRFTLLTAQKPPTEY


    Database link: O95197
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human cerebral cortex tissue. ICC/IF: U-2 OS cells.
  • General notes

     This product was previously labelled as RTN3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human RTN3/HAP protein (ab153066)

Applications

Our Abpromise guarantee covers the use of ab246911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
  • Tissue specificity

    Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
  • Sequence similarities

    Contains 1 reticulon domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Target information above from: UniProt accession O95197 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10313 Human
    • Omim: 604249 Human
    • SwissProt: O95197 Human
    • Unigene: 473761 Human
    • Alternative names

      • ASYIP antibody
      • Neuroendocrine specific protein like 2 antibody
      • Neuroendocrine-specific protein-like 2 antibody
      • Neuroendocrine-specific protein-like II antibody
      • NSP like protein II antibody
      • NSP-like protein 2 antibody
      • NSP-like protein II antibody
      • NSPL2 antibody
      • NSPLII antibody
      • Reticulon 3 antibody
      • Reticulon-3 antibody
      • Reticulon3 antibody
      • Rtn3 antibody
      • RTN3_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (ab246911)
      Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (ab246911)

      PFA-fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for RTN3/HAP using ab246911 (green) at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)

      Paraffin-embedded human cerebral cortex tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)

      Paraffin-embedded human colon tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in glandular cells as expected.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)

      Paraffin-embedded human lymph node tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in lymphoid cells as expected.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)

      Paraffin-embedded human liver tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab246911? Please let us know so that we can cite the reference in this datasheet.

    ab246911 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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