Product nameAnti-RTN3/HAP antibody
See all RTN3/HAP primary antibodies
DescriptionRabbit polyclonal to RTN3/HAP
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RTN3/HAP aa 750-800. The exact sequence is proprietary. AAU81930.1
Database link: O95197
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 113 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
Tissue specificityIsoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
Sequence similaritiesContains 1 reticulon domain.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
- ASYIP antibody
- Neuroendocrine specific protein like 2 antibody
- Neuroendocrine-specific protein-like 2 antibody
All lanes : Anti-RTN3/HAP antibody (ab264314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 30 seconds
RTN3/HAP was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264314 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264314 at 0.4 µg/ml.
Lane 1: ab264314 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling RTN3/HAP with ab264314 at 1/200 dilution.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264314 has not yet been referenced specifically in any publications.