Anti-RTN3/HAP antibody (ab68328)
Key features and details
- Mouse polyclonal to RTN3/HAP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RTN3/HAP antibody
See all RTN3/HAP primary antibodies -
Description
Mouse polyclonal to RTN3/HAP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RTN3/HAP aa 1-236.
Database link: NP_006045.1 -
Positive control
- WB: Wild-type HEK-293T, HeLa, U-87 MG, and RTN3/HAP transfected 293T cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis. -
Tissue specificity
Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma. -
Sequence similarities
Contains 1 reticulon domain. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 10313 Human
- Omim: 604249 Human
- SwissProt: O95197 Human
- Unigene: 473761 Human
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Alternative names
- ASYIP antibody
- Neuroendocrine specific protein like 2 antibody
- Neuroendocrine-specific protein-like 2 antibody
see all
Images
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All lanes : Anti-RTN3/HAP antibody (ab68328) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : RTN3 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : U-87 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-RTN3/HAP antibody staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab68328 was shown to bind specifically to RTN3/HAP. A band was observed at 24 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in RTN3 knockout cell line ab266517 (knockout cell lysate ab258647). To generate this image, wild-type and RTN3 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-RTN3/HAP antibody (ab68328) at 1/500 dilution
Lane 1 : RTN3/HAP transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 26 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (3)
ab68328 has been referenced in 3 publications.
- He M et al. Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology. J Orthop Surg Res 17:177 (2022). PubMed: 35331286
- Li J et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
- Li B et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB . PubMed: 28717245