Overview

  • Product name

  • Description

    Mouse polyclonal to RTN3/HAP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RTN3/HAP aa 1-236.
    Database link: NP_006045.1

  • Positive control

    • RTN3/HAP transfected 293T cell lysate
  • General notes

     This product was previously labelled as RTN3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab68328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
  • Tissue specificity

    Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
  • Sequence similarities

    Contains 1 reticulon domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ASYIP antibody
    • Neuroendocrine specific protein like 2 antibody
    • Neuroendocrine-specific protein-like 2 antibody
    • Neuroendocrine-specific protein-like II antibody
    • NSP like protein II antibody
    • NSP-like protein 2 antibody
    • NSP-like protein II antibody
    • NSPL2 antibody
    • NSPLII antibody
    • Reticulon 3 antibody
    • Reticulon-3 antibody
    • Reticulon3 antibody
    • Rtn3 antibody
    • RTN3_HUMAN antibody
    see all

Images

  • All lanes : Anti-RTN3/HAP antibody (ab68328) at 1/500 dilution

    Lane 1 : RTN3/HAP transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 26 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Li B  et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB . Read more (PubMed: 28717245) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Hela
Blocking step
Fish Skin Gelatin (FSG) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 05 2015

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