Key features and details
- Mouse polyclonal to RTN3/HAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RTN3/HAP antibody
See all RTN3/HAP primary antibodies
DescriptionMouse polyclonal to RTN3/HAP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RTN3/HAP aa 1-236.
Database link: NP_006045.1
- RTN3/HAP transfected 293T cell lysate
This product was previously labelled as RTN3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).|
FunctionMay be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
Tissue specificityIsoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
Sequence similaritiesContains 1 reticulon domain.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
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All lanes : Anti-RTN3/HAP antibody (ab68328) at 1/500 dilution
Lane 1 : RTN3/HAP transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 26 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
ab68328 has been referenced in 1 publication.
- Li B et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB . PubMed: 28717245