Anti-RTR antibody (ab38816)
- Datasheet
- References (4)
- Protocols
Overview
-
Product name
Anti-RTR antibody
See all RTR primary antibodies -
Description
Rabbit polyclonal to RTR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human RTR aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38815) -
Positive control
- Recombinant Human RTR protein (ab114813) can be used as a positive control in WB. This antibody gave a positive signal in Testis (Human) Tissue Lysate - adult normal tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab38816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 22926843 |
Target
-
Function
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. -
Tissue specificity
Shows highest expression in the germ cells of the adult testis. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR6 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2649 Human
- Entrez Gene: 14536 Mouse
- Entrez Gene: 362125 Rat
- Omim: 602778 Human
- SwissProt: Q15406 Human
- SwissProt: Q64249 Mouse
- Unigene: 586460 Human
- Unigene: 439703 Mouse
-
Alternative names
- GCNF 1 antibody
- GCNF antibody
- GCNF1 antibody
see all
Images
-
Western blot - Anti-RTR antibody (ab38816)All lanes : Anti-RTR antibody (ab38816) at 1/250 dilution
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human testis tissue lysate - total protein (ab30257) withHuman RTR peptide (ab38815) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 230 kDa (possible cross reactivity) -
Western blot - Anti-RTR antibody (ab38816)This image is courtesy of an anonymous Abreview.All lanes : Anti-RTR antibody (ab38816) at 1/80 dilution
Lane 1 : Cell extract of HAmGCNF expressing COS1 cells.
Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg
Secondary
All lanes : Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTR antibody (ab38816)This image is courtesy of an Abreview from Zachary Yu-Ching Lin.IHC-P image of RTR staining with ab38816 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab38816 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Datasheets and documents
References
This product has been referenced in:
- Valdmanis PN et al. miR-122 removal in the liver activates imprinted microRNAs and enables more effective microRNA-mediated gene repression. Nat Commun 9:5321 (2018). Read more (PubMed: 30552326) »
- Chalmel F et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Hum Reprod 27:3233-48 (2012). IHC-P ; Human, Rat . Read more (PubMed: 22926843) »
