Anti-RTR antibody (ab38816)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-RTR antibody
See all RTR primary antibodies -
DescriptionRabbit polyclonal to RTR
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human RTR aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38815) -
Positive control
- Recombinant Human RTR protein (ab114813) can be used as a positive control in WB. This antibody gave a positive signal in Testis (Human) Tissue Lysate - adult normal tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab38816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 22926843 |
Target
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FunctionOrphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.
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Tissue specificityShows highest expression in the germ cells of the adult testis.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR6 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2649 Human
- Entrez Gene: 14536 Mouse
- Entrez Gene: 362125 Rat
- Omim: 602778 Human
- SwissProt: Q15406 Human
- SwissProt: Q64249 Mouse
- Unigene: 586460 Human
- Unigene: 439703 Mouse
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Alternative names
- GCNF 1 antibody
- GCNF antibody
- GCNF1 antibody
see all
Images
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Western blot - Anti-RTR antibody (ab38816)All lanes : Anti-RTR antibody (ab38816) at 1/250 dilution
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human testis tissue lysate - total protein (ab30257) withHuman RTR peptide (ab38815) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 230 kDa (possible cross reactivity) -
Western blot - Anti-RTR antibody (ab38816)This image is courtesy of an anonymous Abreview.All lanes : Anti-RTR antibody (ab38816) at 1/80 dilution
Lane 1 : Cell extract of HAmGCNF expressing COS1 cells.
Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg
Secondary
All lanes : Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTR antibody (ab38816)This image is courtesy of an Abreview from Zachary Yu-Ching Lin.IHC-P image of RTR staining with ab38816 on tissue sections from adult mouse testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab38816 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Datasheets and documents
References
This product has been referenced in:
- Chalmel F et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Hum Reprod 27:3233-48 (2012). IHC-P ; Human, Rat . Read more (PubMed: 22926843) »
- Bianco C et al. Regulation of human Cripto-1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells. J Cell Physiol : (2012). WB ; Human . Read more (PubMed: 23129342) »
