Anti-RTR antibody (ab38816)
Key features and details
- Rabbit polyclonal to RTR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RTR antibody -
Description
Rabbit polyclonal to RTR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human RTR aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38815) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815).
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| Notes |
|---|
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WB
1/250. Detects a band of approximately 53 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human RTR peptide (ab38815). |
Target
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Function
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. -
Tissue specificity
Shows highest expression in the germ cells of the adult testis. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR6 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2649 Human
- Entrez Gene: 14536 Mouse
- Entrez Gene: 362125 Rat
- Omim: 602778 Human
- SwissProt: Q15406 Human
- SwissProt: Q64249 Mouse
- Unigene: 586460 Human
- Unigene: 439703 Mouse
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Alternative names
- GCNF 1 antibody
- GCNF antibody
- GCNF1 antibody
see all
Images
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Western blot - Anti-RTR antibody (ab38816)All lanes : Anti-RTR antibody (ab38816) at 1/250 dilution
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human testis tissue lysate - total protein (ab30257) withHuman RTR peptide (ab38815) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Additional bands at: 230 kDa (possible cross reactivity) -
Western blot - Anti-RTR antibody (ab38816)This image is courtesy of an anonymous Abreview.All lanes : Anti-RTR antibody (ab38816) at 1/80 dilution
Lane 1 : Cell extract of HAmGCNF expressing COS-1 cells.
Lane 2 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 20 µg
Lane 3 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 20 µg
Lane 4 : Nuclear extract of Dox+ control mES cells treated with RA for 1.5 days. at 40 µg
Lane 5 : Nuclear extract of Dox- biohGCNF expressing mES cells treated with RA for 1.5 days. at 40 µg
Secondary
All lanes : Goat anti-rabbit (Santa Cruz) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Datasheets and documents
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Datasheet download
References (4)
ab38816 has been referenced in 4 publications.
- Valdmanis PN et al. miR-122 removal in the liver activates imprinted microRNAs and enables more effective microRNA-mediated gene repression. Nat Commun 9:5321 (2018). PubMed: 30552326
- Chalmel F et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Hum Reprod 27:3233-48 (2012). IHC-P ; Human, Rat . PubMed: 22926843
- Bianco C et al. Regulation of human Cripto-1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells. J Cell Physiol : (2012). WB ; Human . PubMed: 23129342
- Zuccotti M et al. Oct-4 regulates the expression of Stella and Foxj2 at the Nanog locus: implications for the developmental competence of mouse oocytes. Hum Reprod 24:2225-37 (2009). ICC/IF ; Mouse . PubMed: 19477878
