Overview

  • Product name

    Anti-RTT106 antibody
  • Description

    Rabbit polyclonal to RTT106
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae RTT106 aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: P40161

  • Positive control

    • This antibody gave a positive signal in both Wild Type Exponentially growing yeast and W303 Saccromyces cerevisiae.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Involved in regulation of Ty1 transposition.
  • Sequence similarities

    Belongs to the RTT106 family.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

    • Alternative names

      • Histone chaperone rtt106 antibody
      • Regulator of Ty1 transposition protein 106 antibody
      • RT106_YEAST antibody
      • rtt106 antibody

    Images

    • All lanes : Anti-RTT106 antibody (ab130122) at 1/250 dilution (Milk blocking 3%)

      Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
      Lane 2 : W303 Yeast Lysate Whole Cell Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 52 kDa
      Observed band size: 49 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 16 kDa (possible non-specific binding)


      Exposure time: 20 minutes


      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab130122 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    References

    ab130122 has not yet been referenced specifically in any publications.

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