Product nameAnti-Rubella Virus capsid antibody [9B11]
DescriptionMouse monoclonal [9B11] to Rubella Virus capsid
Tested applicationsSuitable for: Indirect ELISA, WBmore details
Species reactivityReacts with: Rubella virus
Recombinant full length protein corresponding to Rubella Virus capsid.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR120838-1 and GR210838-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab34749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/1000 - 1/10000.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 115 kDa.|
RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
Cellular localizationCytoplasmic in host cells concentrated around Golgi region and mitochondrion.
- Coat protein antibody
- p110 antibody
- rubella capsid antibody
- Structural polyprotein antibody
All lanes : Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/ml
Lane 1 : Prestained M.Wt markers
Lane 2 : crude yeast cell lysate [mock control]
Lanes 3-4 : irrelevant viral proteins
Lane 5 : recombinant purified rubella capsid protein
Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein
Predicted band size: 115 kDa
This product has been referenced in:
- Trinh QD et al. Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro. Viruses 10:N/A (2018). Read more (PubMed: 29300335) »
- Neven B et al. Cutaneous and Visceral Chronic Granulomatous Disease Triggered by a Rubella Virus Vaccine Strain in Children With Primary Immunodeficiencies. Clin Infect Dis 64:83-86 (2017). IHC . Read more (PubMed: 27810866) »