Key features and details
- Mouse monoclonal [9B11] to Rubella Virus capsid
- Suitable for: Indirect ELISA, WB
- Reacts with: Rubella virus
- Isotype: IgG1
Product nameAnti-Rubella Virus capsid antibody [9B11]
DescriptionMouse monoclonal [9B11] to Rubella Virus capsid
Tested applicationsSuitable for: Indirect ELISA, WBmore details
Species reactivityReacts with: Rubella virus
Recombinant full length protein corresponding to Rubella Virus capsid.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR120838-1 and GR210838-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab34749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/1000 - 1/10000.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 115 kDa.|
RelevanceRubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.
Cellular localizationCytoplasmic in host cells concentrated around Golgi region and mitochondrion.
- Coat protein antibody
- p110 antibody
- rubella capsid antibody
- Structural polyprotein antibody
All lanes : Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/ml
Lane 1 : Prestained M.Wt markers
Lane 2 : crude yeast cell lysate [mock control]
Lanes 3-4 : irrelevant viral proteins
Lane 5 : recombinant purified rubella capsid protein
Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein
Predicted band size: 115 kDa
ab34749 has been referenced in 7 publications.
- Trinh QD et al. Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro. Viruses 10:N/A (2018). PubMed: 29300335
- Sun AC et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
- Neven B et al. Cutaneous and Visceral Chronic Granulomatous Disease Triggered by a Rubella Virus Vaccine Strain in Children With Primary Immunodeficiencies. Clin Infect Dis 64:83-86 (2017). IHC . PubMed: 27810866
- Aoki Y et al. Chance Isolation of Non-Pathogenic Vaccine-Derived Measles and Rubella Viruses from Children with Acute Respiratory Infections. Jpn J Infect Dis 69:350-1 (2016). PubMed: 26902222
- Perelygina L et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF . PubMed: 27613149
- Perelygina L et al. Differences in Establishment of Persistence of Vaccine and Wild Type Rubella Viruses in Fetal Endothelial Cells. PLoS One 10:e0133267 (2015). PubMed: 26177032
- Park B et al. Proteolytic cleavage in an endolysosomal compartment is required for activation of Toll-like receptor 9. Nat Immunol 9:1407-14 (2008). PubMed: 18931679