Anti-Rubella Virus capsid antibody [9B11] (ab34749)
![Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)](https://www.abcam.com/ps/products/34/ab34749/Images/ab34749-47965-anti-rubella-virus-capsid-antibody-9b11-western-blot.jpg "Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)")
Key features and details
- Mouse monoclonal [9B11] to Rubella Virus capsid
- Suitable for: Indirect ELISA, WB
- Reacts with: Rubella virus
- Isotype: IgG1
Overview
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Product name
Anti-Rubella Virus capsid antibody [9B11] -
Description
Mouse monoclonal [9B11] to Rubella Virus capsid -
Host species
Mouse -
Tested applications
Suitable for: Indirect ELISA, WBmore details -
Species reactivity
Reacts with: Rubella virus -
Immunogen
Recombinant full length protein corresponding to Rubella Virus capsid.
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General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR120838-1 and GR210838-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9B11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA |
1/1000 - 1/10000.
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| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 115 kDa.
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| Notes |
|---|
|
Indirect ELISA
1/1000 - 1/10000. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 115 kDa. |
Target
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Relevance
Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase. -
Cellular localization
Cytoplasmic in host cells concentrated around Golgi region and mitochondrion. -
Alternative names
- Coat protein antibody
- p110 antibody
- rubella capsid antibody
- Structural polyprotein antibody
Images
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Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)All lanes : Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/ml
Lane 1 : Prestained M.Wt markers
Lane 2 : crude yeast cell lysate [mock control]
Lanes 3-4 : irrelevant viral proteins
Lane 5 : recombinant purified rubella capsid protein
Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein
Predicted band size: 115 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab34749 has been referenced in 8 publications.
- Pham NTK et al. The Epithelial-to-Mesenchymal Transition-Like Process Induced by TGF-ß1 Enhances Rubella Virus Binding and Infection in A549 Cells via the Smad Pathway. Microorganisms 9:N/A (2021). PubMed: 33806778
- Sun AC et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
- Trinh QD et al. Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro. Viruses 10:N/A (2018). PubMed: 29300335
- Neven B et al. Cutaneous and Visceral Chronic Granulomatous Disease Triggered by a Rubella Virus Vaccine Strain in Children With Primary Immunodeficiencies. Clin Infect Dis 64:83-86 (2017). IHC . PubMed: 27810866
- Aoki Y et al. Chance Isolation of Non-Pathogenic Vaccine-Derived Measles and Rubella Viruses from Children with Acute Respiratory Infections. Jpn J Infect Dis 69:350-1 (2016). PubMed: 26902222
- Perelygina L et al. Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies. J Allergy Clin Immunol 138:1436-1439.e11 (2016). IF . PubMed: 27613149
- Perelygina L et al. Differences in Establishment of Persistence of Vaccine and Wild Type Rubella Viruses in Fetal Endothelial Cells. PLoS One 10:e0133267 (2015). PubMed: 26177032
- Park B et al. Proteolytic cleavage in an endolysosomal compartment is required for activation of Toll-like receptor 9. Nat Immunol 9:1407-14 (2008). PubMed: 18931679