Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Product nameRubella Virus IgG ELISA kit (avidity)
See all Rubella Virus IgG kits
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Rubella Virus IgG ELISA kit (avidity) (ab247202) is designed to indicate the Rubella-specific IgG avidity in human serum or plasma (citrate, heparin) to differentiate between acute and past infection.
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample (dual pipetting). After washing the wells to remove all unbound sample material, one well is incubated with avidity reagent and the corresponding well with washing buffer. The avidity reagent removes the low-avidity antibodies from the antigens whereas the high-avidity ones are still bound to the specific antigens. After a second washing step to remove the rest of avidity reagent and low-avidity antibodies, a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a third washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
The presence of IgG antibodies to Rubella Virus indicates the occurrence of the infection but does not distinguish between recent and past infection. Virus-specific IgM antibodies are first detected approximately in ten days and peak at about four weeks post infection. They may persist for several months after acute infections. Based on the evidence that antibody avidity gradually increases after exposure to an immunogen, avidity of IgG antibodies can be used as a marker for distinguishing recent primary from long-term infections. Avidity describes the binding strength of a specific antibody to its antigen. Low-avidity IgG antibodies indicate a primary infection, whereas the presence of IgG antibodies with high avidity points to persistency or reactivation of infection.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests Avidity Reagent 1 x 15ml Cover Foil 1 unit Empty 20ml bottle for Washing buffer 1x dilution 1 unit IgG high Control 1 x 2ml IgG low Control 1 x 2ml IgG Sample Diluent 1 x 100ml Rubella Virus anti-IgG Conjugate 1 x 20ml Rubella Virus Coated Microplate (IgG) 1 unit Rubella Virus IgG Standard A 1 x 2ml Rubella Virus IgG Standard B 1 x 2ml Rubella Virus IgG Standard C 1 x 2ml Rubella Virus IgG Standards D 1 x 2ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml Washing Buffer 1 x 50ml
ab247202 has not yet been referenced specifically in any publications.