RuBi-4AP, Caged K+ channel blocker (ab120410)

Submit a review Q&A (7)References (1)
Chemical Structure - RuBi-4AP, Caged K<sup>+</sup> channel blocker (ab120410)

    Key features and details

    • Caged K+ channel blocker, uncaged by visible light
    • CAS Number:
    • Soluble in water
    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      RuBi-4AP, Caged K+ channel blocker

    • Description

      Caged K+ channel blocker, uncaged by visible light

    • Biological description

      Water soluble caged 4-aminopyridine (4-AP) based on ruthenium photochemistry. Excited by visible wavelengths and has two-photon uncaging capabilities under physiological conditions. Releases voltage-dependent K+ channel blocker 4-aminopyridine (ab120122) when uncaged.

    • General notes

      RuBi-4AP is light sensitive and it is recommended that the compound is protected from light.

      Sold under license from Columbia University; Patent Pending

    • Chemical structure

      Chemical Structure

    Properties

    Associated products

    Images

    • Chemical Structure - RuBi-4AP, Caged K<sup>+</sup> channel blocker (ab120410)
      Chemical Structure - RuBi-4AP, Caged K+ channel blocker (ab120410)
      2D chemical structure image of ab120410, RuBi-4AP, Caged K+ channel blocker

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab120410? Please let us know so that we can cite the reference in this datasheet.

    ab120410 has been referenced in 1 publication.

    • Rowan MJ  et al. Distinct Kv channel subtypes contribute to differences in spike signaling properties in the axon initial segment and presynaptic boutons of cerebellar interneurons. J Neurosci 34:6611-23 (2014). PubMed: 24806686

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Question

    Thank you for your prompt action. 4-AP has a very potent effect on the channels we are studying, therefore even low levels of non-caged compound are strongly inhibited.
    I would prefer to wait for the replacement.
    Should I refer any queries about this order to us.technical@abcam.com?
    Thank you again for the quick action.

    Read More
    Answer

    Thank you for your patience and understanding in this matter.
    Once the new batch of RuBi-4AP is ready, I will send on 3 replacement vials to you. If you have any other questions or concerns in the meantime, please feel free to drop me an email.
    I hope you have a great weekend.

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    Question

    Hello,
    I would like to order the remaining vials of RuBi-4AP from lot APN12359-1-1 at this time. The product is working very nicely. How can I best expedite this process?
    Thanks,

    Read More
    Answer

    I now have further information to share with you in regards to the RuBi-4AP (ab120410). We do not have any further stock of lot number APN12359-1-1 available other than the 7 vials you have placed an order for.
    We will however be producing more of the compound. I can understand why you would be concerned over the purity of this new batch but please let me reassure you that we will be carrying out stringent quality control testing to make sure that the impurity that was identified with the previous lot you purchased is not present.
    I would suggest placing an order for one vial when the new lot comes into stock and if you are not happy with it we would refund you.
    I hope this information has been of help. If I can be of any further assistance please do let me know.
    Until then, I wish you all the best with your research.

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    Question

    Hello,
    I would like to order the remaining vials of RuBi-4AP from lot APN12359-1-1 at this time. The product is working very nicely. How can I best expedite this process?
    Thanks,

    Read More
    Answer

    Thank you for your email.
    I am pleased to hear that you have found the RuBi-4AP (ab120410) to work well for you.
    We currently have a total of 7 vials of the lot number APN12359-1-1 in stock (7x10 mg). As you are ordering using a credit card you will need to order either by calling and placing an order (Call (888) 77-ABCAM (22226)) or placing an order online.
    When placing the order, please specify the lot number you are interested in. I am currently requesting further information from the lab as to if we have any further compound of the same lot number available. I will get back to you with this information as soon as I have it.

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    Question

    Hi,
    After some initial testing on the new RuBi-4AP compound, it appears that this lot (#APN12359-1-1) is pure and working well. I thank you for your efforts in getting this
    material to a high standard. How many vials of this lot are now currently available? We may be interested in purchasing more now.
    Thanks,

    Read More
    Answer

    Thank you for getting back to us with that information.
    I am glad to hear the new RuBi-4AP (ab120410) has worked well for you. We currently have a further 3 vials (3x10 mg) of the same lot, APN12359-1-1, in stock. We would expect to have further material in stock, however this would be of a different lot number. We would however make sure to test this new lot with the same analyses which revealed the impurity in the lot you had problems with.
    I hope this information has been of help. If you have any further questions please do not hesitate to ask.

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    Question

    Hi,
    Please send the replacement vials(RuBi-4AP, ab120410) at your earliest convenience!
    Thanks,

    Read More
    Answer

    Thank you for that confirmation.
    I have arranged for a new order of 3 vials of RuBi-4AP to be sent to you (free of charge) as soon as we have it available. This is on the order number xxxxxx. If you have any questions regarding the status of the order please quote this number.
    I am sorry for the delay and any inconvenience this is causing you.

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    Question

    I have encountered a problem with the Rubi-4AP I ordered (*). This is a 'caged' compound.
    It appears that the compound is not well caged in this batch. Although the compound was never exposed to light,
    results with the caged Rubi-4AP are similar to that with 4-AP itself.
    I would appreciate a replacement order (3 vials, 10mg) from a new batch. Please don’t hesitate to contact me
    with any concerns.

    Read More
    Answer

    Thank you for your patience in this matter/
    Following analysis we have some more information about this compound. It appears that low levels of 4-AP (about 6%) have been detected in the sample. We are in the process of improving the purity and would like to thank the custom you for bringing this matter to our attention.

    We envisage the purification will take three to four weeks. Therefore, I can go ahead and refund the cost of the original purchase, or if you would like to wait for the new batch to be produced, I can send you replacement vials then.
    Please let me know, how you would like to proceed.
    We would like to apologies for any inconvenience this may have caused.

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    Question

    I have encountered a problem with the Rubi-4AP I ordered (Batch *). This is a 'caged' compound.
    It appears that the compound is not well caged in this batch. Although the compound was never exposed to light,
    results with the caged Rubi-4AP are similar to that with 4-AP itself.
    I would appreciate a replacement order (3 vials, 10mg) from a new batch. Please don’t hesitate to contact me

    Read More
    Answer

    Thank you for contacting Abcam.
    I am sorry about the issues you are having with ab120410. We are currently looking into this issue and checking our current stock, so that we can be sure that what we have, is working as it should.
    Once I hear back from the lab and we know that are current stocks are ok, I will contact you and then arrange for the replacements to be sent.
    I do appreciate your patience in this matter.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
    For licensing inquiries, please contact partnerships@abcam.com