Overview

  • Product name

    RuBi-Glutamate, caged-glutamate compound
  • Description

    Novel caged-glutamate compound, uncaged by visible light
  • Biological description

    Novel caged-glutamate compound based on ruthenium photochemistry. Excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations. Enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single cell, or even single spine, precision.

    Also available in simple stock solutions (ab146718) - add 1 ml of water to get an exact, ready-to-use concentration.
  • General notes

    RuBi-Glutamate is light sensitive and it is recommended that the compound is protected from light.

    Sold under license from Columbia University; Patent Pending.

  • Chemical structure

    Chemical Structure

Properties

References

This product has been referenced in:

  • Salierno M  et al. A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes. J Inorg Biochem 104:418-22 (2010). Read more (PubMed: 20060592) »
  • Fino E  et al. RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines. Front Neural Circuits 3:2 (2009). Read more (PubMed: 19506708) »

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

Thank you for contacting us. The following is the email I have received from my colleagues.

Regarding the stability - we should advise the customer that in a RT solution the compound shows partial decomposition to give a small amount of un-caged glutamate after 24 hours (even when shielded from the light). If handled according to the datasheet then this should not happen.

They have confirmed that the product is little unstable when in solution so recommended to be used in 24 hours.

I have also forwarded your contact details to product developer you will be receiving a call soon.

We will be sending one 10 mg pack of this product tomorrow morning.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for confirming that information.

I will arrange for 3 vials of RuBi-Glutamate (ab120408) from a different lot number to be sent out to you. This is currently on the order number 1148036but please be aware that it may be moved to a different order through consolidation. If you would like to track the item please quote xxxxxx to any of our customer service representatives.

In order to investigate this case further, it would be very helpful to know which of the 4 vials there was a problem with and which one worked. Did the customer receive all the vials at the same time or were 3 sent and then 1 separately, or vice versa? Do the customers know which one it was that worked?

Am I correct in thinking that the customer observed a reaction with the compound without applying any light (suggesting that the compound was uncaged before applying)?

Would the customer mind describing how the product was stored and what tests were performed with the compound? This will allow us to understand what may have been contributing to the problems.

Many thanks in advance for your cooperation in this case.

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Answer

Thank you for contacting us.

The former Ascent lab (now Abcam Biochemicals) got back to me. They are looking into the issue you have encountered and possible explanations. I have asked them to contact you directly, and my colleague said that either he himself or our colleague in Scientific Support at Abcam's xxx office will get in touch with you very soon to resolve the problem.

I wish you all the best and that an explanation can be found quickly.
Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for your call today and for letting us know about the trouble with ab120408.

As we discussed, you can forward all of the details about the problem to me, and I will get in touch with my colleagues at Abcam Biochemicals (formally Ascent Scientific)in order to help resolve this issue.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

Thank you for getting back to me.

I am sorry that I was not able to reply to your email sooner. I have been away from the office for the week. I am very pleased to hear that you have found the newly purified compound to work well in your experiments. I really appreciate all the help you have provided us in trying to resolve this issue. It really has been invaluable.

I would be very much appreciate if you could provide me with the figure and a few details of your experiment. Would you mind if I add this information to the datasheet of the compound? I think this would be very useful to our other customers.

I look forward to hearing from you.

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Answer

Thank you for your message which has been forwarded to me as my colleague ##### is currently away from the office.

I am very pleased to hear the replacement is working well. We appreciate you contacting us to let us know. I will ensure ##### has a copy of your message when she returns to the office next week.

Good luck with your experiments and if you have any further questions or concerns, please do not hesitate to contact us.

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Answer

Thank you for your very kind reply.

We do try to support our products to the best of our abilities and it is nice to know that it is appreciated. I really do hope you will find the new compound to work well in your experiments and the data you have offered to share would be tremendously valuable.

As I said, the new batch should be with you next week. I'll let you know if thereare any further developments.

Until then, I wish you all the best with your research.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

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