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I have tried the new compound you sent and it works beautifully. I can detect no electrophysiological response in the cell when I puff on theRuBi until I engage the laser. I very do much appreciate your help in this matter, and am glad that it is finally resolved. I will create a figure of an example experiment with glutamate uncaging at a single spine.
Asked on Jul 16 2012
Thank you for getting back to me.
I am sorry that I was not able to reply to your email sooner. I have been away from the office for the week. I am very pleased to hear that you have found the newly purified compound to work well in your experiments. I really appreciate all the help you have provided us in trying to resolve this issue. It really has been invaluable.
I would be very much appreciate if you could provide me with the figure and a few details of your experiment. Would you mind if I add this information to the datasheet of the compound? I think this would be very useful to our other customers.
I look forward to hearing from you.
Answered on Jul 16 2012