Product nameAnti-Rubicon/Baron antibody
See all Rubicon/Baron primary antibodies
DescriptionRabbit polyclonal to Rubicon/Baron
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Mouse Rubicon/Baron aa 750-850 conjugated to keyhole limpet haemocyanin.
Database link: Q80U62
- This antibody gave a positive signal in the following tissue lysates: Mouse Liver; Mouse Kidney; Human Liver; Human Kidney. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
This product was previously labelled as Rubicon
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab156052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
- Information by UniProt
- Baron antibody
- Beclin 1 associated RUN domain containing protein antibody
- Beclin-1 associated RUN domain containing protein antibody
ICC/IF image of ab156052 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab156052 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h.
ab50533 (Mouse monoclonal [Rab7-117] to RAB7) was also incubated along with ab156052 to demonstrate antigen co-localization. Signal detected using Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed (ab150120) (pseudo-colored red) DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-Rubicon/Baron antibody (ab156052) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Additional bands at: 42 kDa (possible non-specific binding), 48 kDa (possible non-specific binding)
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156052 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.