Product nameAnti-Rubicon/Baron antibody
See all Rubicon/Baron primary antibodies
DescriptionRabbit polyclonal to Rubicon/Baron
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to Human Rubicon/Baron aa 922-972.
Database link: NP_055502.1
- HeLa whole cell lysate (ab150035)
This product was previously labelled as Rubicon
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab92388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 109 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22843690|
- Information by UniProt
- Baron antibody
- Beclin 1 associated RUN domain containing protein antibody
- Beclin-1 associated RUN domain containing protein antibody
All lanes : Anti-Rubicon/Baron antibody (ab92388) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 3 minutes
Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG.
Detection: chemiluminescence with exposure time of 30 seconds.
ICC/IF image of ab92388 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92388, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of Human renal cancer tissue, staining Rubicon/Baron with ab92388. Tissue was fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
Immunohistochemical analysis of Human renal tumour cells, staining Rubicon/Baron with ab92388. Cells were fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- Aikawa C et al. NLRX1 Negatively Regulates Group A Streptococcus Invasion and Autophagy Induction by Interacting With the Beclin 1-UVRAG Complex. Front Cell Infect Microbiol 8:403 (2018). Read more (PubMed: 30488027) »
- Su H et al. VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy. Mol Cell 67:907-921.e7 (2017). Read more (PubMed: 28844862) »