Overview

  • Product name

    Anti-Rubicon/Baron antibody
    See all Rubicon/Baron primary antibodies
  • Description

    Rabbit polyclonal to Rubicon/Baron
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Rubicon/Baron aa 922-972.
    Database link: NP_055502.1

  • Positive control

  • General notes

     This product was previously labelled as Rubicon

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 22843690

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • Baron antibody
    • Beclin 1 associated RUN domain containing protein antibody
    • Beclin-1 associated RUN domain containing protein antibody
    • hypothetical protein LOC9711 antibody
    • Kiaa0226 antibody
    • OTTHUMP00000208021 antibody
    • OTTHUMP00000208023 antibody
    • OTTHUMP00000208024 antibody
    • RUBIC_HUMAN antibody
    • Rubicon antibody
    • RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody
    • Run domain Beclin 1 interacting and cystein rich containing protein antibody
    • Run domain Beclin-1 interacting and cysteine-rich containing protein antibody
    • RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody
    see all

Images

  • All lanes : Anti-Rubicon/Baron antibody (ab92388) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 109 kDa


    Exposure time: 3 minutes
  • Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG.
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ICC/IF image of ab92388 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92388, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of Human renal cancer tissue, staining Rubicon/Baron with ab92388. Tissue was fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemical analysis of Human renal tumour cells, staining Rubicon/Baron with ab92388. Cells were fixed with acetone and incubated with primary antibody. A Cy3-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:

  • Aikawa C  et al. NLRX1 Negatively Regulates Group A Streptococcus Invasion and Autophagy Induction by Interacting With the Beclin 1-UVRAG Complex. Front Cell Infect Microbiol 8:403 (2018). Read more (PubMed: 30488027) »
  • Su H  et al. VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy. Mol Cell 67:907-921.e7 (2017). Read more (PubMed: 28844862) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 20 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Microglia/astrocyte)
Permeabilization
Yes - 0.3% Troton X-100
Specification
Microglia/astrocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 20 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kiney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kiney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 01 2016

Answer

Unfortunately based on sequence alignment of the immunogen against zebrafish Rubicon, AB92388 does not qualify for the AbTrial program. This antibody has only 59% homology where we require 85% or higher.

Read More

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