Anti-Rubicon/Baron antibody (ab92388)
Key features and details
- Rabbit polyclonal to Rubicon/Baron
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rubicon/Baron antibody
See all Rubicon/Baron primary antibodies -
Description
Rabbit polyclonal to Rubicon/Baron -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human Rubicon/Baron aa 922-972.
Database link: NP_055502.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 109 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 9711 Human
- Omim: 613516 Human
- SwissProt: Q92622 Human
- Unigene: 478868 Human
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Alternative names
- Baron antibody
- Beclin 1 associated RUN domain containing protein antibody
- Beclin-1 associated RUN domain containing protein antibody
see all
Images
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All lanes : Anti-Rubicon/Baron antibody (ab92388) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 3 minutes -
Sample: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
Antibodies: ab92388 used for WB 1 µg/ml (lane 1) and used for IP at 3 µg/mg lysate. Lane 2: control IgG.
Detection: chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab92388 has been referenced in 12 publications.
- Peña-Oyarzun D et al. PKD2/polycystin-2 induces autophagy by forming a complex with BECN1. Autophagy 17:1714-1728 (2021). PubMed: 32543276
- Li Y et al. Podocyte EGFR Inhibits Autophagy Through Upregulation of Rubicon in Type 2 Diabetic Nephropathy. Diabetes 70:562-576 (2021). PubMed: 33239448
- Li X et al. CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy 17:4323-4340 (2021). PubMed: 33977871
- Asare PF et al. Inhibition of LC3-associated phagocytosis in COPD and in response to cigarette smoke. Ther Adv Respir Dis 15:17534666211039769 (2021). PubMed: 34852704
- Kim YR et al. Identification of highly potent and selective inhibitor, TIPTP, of the p22phox-Rubicon axis as a therapeutic agent for rheumatoid arthritis. Sci Rep 10:4570 (2020). PubMed: 32165681