Overview

  • Product name

    Anti-Rubicon/Baron antibody - C-terminal
    See all Rubicon/Baron primary antibodies
  • Description

    Rabbit polyclonal to Rubicon/Baron - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment within Human Rubicon/Baron (C terminal). The exact sequence is proprietary.
    Database link: Q92622

  • Positive control

    • WB: NTERA-2 cl.D1 [N2/D1] and Rubicon/Baron-transfected HEK-293T whole cell extracts. IP: Rubicon/Baron-transfected HEK-293T whole cell extract.
  • General notes

     This product was previously labelled as Rubicon

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 109 kDa.
IP 1/100 - 1/500.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • Baron antibody
    • Beclin 1 associated RUN domain containing protein antibody
    • Beclin-1 associated RUN domain containing protein antibody
    • hypothetical protein LOC9711 antibody
    • Kiaa0226 antibody
    • OTTHUMP00000208021 antibody
    • OTTHUMP00000208023 antibody
    • OTTHUMP00000208024 antibody
    • RUBIC_HUMAN antibody
    • Rubicon antibody
    • RUN domain and cysteine rich domain containing, Beclin 1 interacting protein antibody
    • Run domain Beclin 1 interacting and cystein rich containing protein antibody
    • Run domain Beclin-1 interacting and cysteine-rich containing protein antibody
    • RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody
    see all

Images

  • Anti-Rubicon/Baron antibody - C-terminal (ab229499) at 1/1000 dilution + NTERA-2 cl.D1 [N2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 109 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-Rubicon/Baron antibody - C-terminal (ab229499) at 1/20000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Rubicon/Baron, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 109 kDa



    5% SDS-PAGE gel.

  • Rubicon/Baron was immunoprecipitated from Rubicon/Baron-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 4 µg of ab229499. Western blot was performed from the immunoprecipitate using ab229499 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Rubicon/Baron transfected HEK-293T whole cell extract 30 µg (Input). 
    Lane 2: 4 µg preimmune Rabbit IgG instead of ab229499 in Rubicon transfected HEK-293T whole cell extract. 
    Lane 3: ab229499 IP in Rubicon transfected HEK-293T whole cell extract. 

References

ab229499 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229499.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up