Anti-RUBISCO antibody (ab226002)
Key features and details
- Rabbit polyclonal to RUBISCO
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-RUBISCO antibody -
Description
Rabbit polyclonal to RUBISCO -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Soybean -
Immunogen
Recombinant full length protein corresponding to Soybean RUBISCO aa 1 to the C-terminus.
Database link: P27066 -
Positive control
- WB: Recombinant Soybean RUBISCO protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. -
Sequence similarities
Belongs to the RuBisCO large chain family. Type I subfamily. -
Post-translational
modificationsThe disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. -
Cellular localization
Plastid > chloroplast. - Information by UniProt
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Database links
- SwissProt: P27066 Soybean
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Alternative names
- 3 phospho D glycerate carboxylase antibody
- rbcL antibody
- RBL_SPIOL antibody
see all
Images
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All lanes : Anti-RUBISCO antibody (ab226002) at 1/500 dilution
Lane 1 : Recombinant Soybean RUBISCO protein at 0.08 µg
Lane 2 : Recombinant Soybean RUBISCO protein at 0.04 µg
Lane 3 : Recombinant Soybean RUBISCO protein at 0.02 µg
Lane 4 : Recombinant Soybean RUBISCO protein at 0.01 µg
Predicted band size: 53 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226002 has not yet been referenced specifically in any publications.