Key features and details
- Rabbit polyclonal to RUBISCO
- Suitable for: WB
- Isotype: IgG
Product nameAnti-RUBISCO antibody
See all RUBISCO primary antibodies
DescriptionRabbit polyclonal to RUBISCO
Tested applicationsSuitable for: WBmore details
Predicted to work with: Soybean
Recombinant full length protein corresponding to Soybean RUBISCO aa 3-475.
PQTETKASVGFKAGVKDYKLTYYTPDYETKDTDILAAFRVTPQPGVPPEE AGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYGLEPVAGEENQYIAYVA YPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPTAYIKTFQGPP HGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKD DENVNSQPFMRWRDRFLFCAEAIFKSQAETGEIKGHYLNATAGTCEEMMK RAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVID RQKNHGMHFRVLAKALRLSGGDHVHAGTVVGKLEGEREITLGFVDLLRDD FVEKDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQF GGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNEIIREASKWS PELAAACEVWKEIKFEFEAMDTL
Database link: P27066
- WB: Recombinant Soybean RUBISCO protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab226002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 53 kDa).|
FunctionRuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
Sequence similaritiesBelongs to the RuBisCO large chain family. Type I subfamily.
modificationsThe disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
Cellular localizationPlastid > chloroplast.
- Information by UniProt
- SwissProt: P27066 Soybean
- 3 phospho D glycerate carboxylase antibody
- rbcL antibody
- RBL_SPIOL antibody
All lanes : Anti-RUBISCO antibody (ab226002) at 1/500 dilution
Lane 1 : Recombinant Soybean RUBISCO protein at 0.08 µg
Lane 2 : Recombinant Soybean RUBISCO protein at 0.04 µg
Lane 3 : Recombinant Soybean RUBISCO protein at 0.02 µg
Lane 4 : Recombinant Soybean RUBISCO protein at 0.01 µg
Predicted band size: 53 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226002 has not yet been referenced specifically in any publications.