Key features and details
- Chicken polyclonal to RUBISCO
- Suitable for: WB
- Reacts with: Spinacia oleracea
- Isotype: IgY
Product nameAnti-RUBISCO antibody
See all RUBISCO primary antibodies
DescriptionChicken polyclonal to RUBISCO
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Spinacia oleracea
Predicted to work with: Plants
Full length protein; RUBISCO small subunit (Spinach). NCBI Acc : AAC29137.1, Swiss-Prot Q9TIM3.
- Purified spinach RUBISCO.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 15, 45 kDa (predicted molecular weight: 55 kDa).|
FunctionRuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
Sequence similaritiesBelongs to the RuBisCO large chain family. Type I subfamily.
modificationsThe disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
Cellular localizationPlastid > chloroplast.
- Information by UniProt
- 3 phospho D glycerate carboxylase antibody
- rbcL antibody
- RBL_SPIOL antibody
Anti-RUBISCO antibody (ab62391) at 1/2000 dilution + Purified spinach RUBISCO protein at 1 µg
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 15,45 kDa why is the actual band size different from the predicted?
ab62391 has been referenced in 1 publication.
- Cai Z et al. Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco. Protein Cell 5:552-62 (2014). WB . PubMed: 24870149