Overview

  • Product name
  • Description
    Rabbit polyclonal to RUFY1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein fragment, corresponding to an internal region within amino acids 210-529 of Human RUFY1 isoform 2 (UniProt ID: Q96T51-2).

  • Positive control
    • NT2D1, IMR32, U 87 MG and MCF7 cell lysates; MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
  • Tissue specificity
    Broadly expressed, with highest levels in lung, testis, kidney and brain.
  • Sequence similarities
    Contains 1 FYVE-type zinc finger.
    Contains 1 RUN domain.
  • Domain
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications
    Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3000002E04Rik antibody
    • FLJ22251 antibody
    • FYVE finger protein EIP1 antibody
    • FYVE-finger protein EIP1 antibody
    • La binding protein 1 antibody
    • La-binding protein 1 antibody
    • OTTHUMP00000161521 antibody
    • Rab4 interacting protein antibody
    • Rab4-interacting protein antibody
    • RABIP4 antibody
    • RUFY1 antibody
    • RUFY1_HUMAN antibody
    • RUN and FYVE domain containing 1 antibody
    • RUN and FYVE domain-containing protein 1 antibody
    • RUN and FYVE domains containing protein 1 antibody
    • ZFYVE12 antibody
    • Zinc finger FYVE domain containing protein 12 antibody
    • Zinc finger FYVE domain-containing protein 12 antibody
    see all

Images

  • Anti-RUFY1 antibody (ab155217) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 69 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed MCF7 cells, labeling RUFY1 using ab155217 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155217.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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