Key features and details
- Rabbit polyclonal to RUFY1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RUFY1 antibody
See all RUFY1 primary antibodies
DescriptionRabbit polyclonal to RUFY1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant protein fragment, corresponding to an internal region within amino acids 210-529 of Human RUFY1 isoform 2 (UniProt ID: Q96T51-2).
- NT2D1, IMR32, U 87 MG and MCF7 cell lysates; MCF7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 69 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Tissue specificityBroadly expressed, with highest levels in lung, testis, kidney and brain.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RUN domain.
DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
modificationsPhosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- 3000002E04Rik antibody
- FLJ22251 antibody
- FYVE finger protein EIP1 antibody
ab155217 has not yet been referenced specifically in any publications.