Overview

  • Product name

  • Description

    Goat polyclonal to RUFY1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human RUFY1 aa 479-491 (Cysteine residue). (NP_079434.3).
    Sequence:

    C-QKNEAITSFEGKT


    Database link: Q96T51

  • Positive control

    • IHC-P: Human small intestine tissue. WB: HeLa cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 80 kDa.

Target

  • Function

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
  • Tissue specificity

    Broadly expressed, with highest levels in lung, testis, kidney and brain.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
    Contains 1 RUN domain.
  • Domain

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications

    Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3000002E04Rik antibody
    • FLJ22251 antibody
    • FYVE finger protein EIP1 antibody
    • FYVE-finger protein EIP1 antibody
    • La binding protein 1 antibody
    • La-binding protein 1 antibody
    • OTTHUMP00000161521 antibody
    • Rab4 interacting protein antibody
    • Rab4-interacting protein antibody
    • RABIP4 antibody
    • RUFY1 antibody
    • RUFY1_HUMAN antibody
    • RUN and FYVE domain containing 1 antibody
    • RUN and FYVE domain-containing protein 1 antibody
    • RUN and FYVE domains containing protein 1 antibody
    • ZFYVE12 antibody
    • Zinc finger FYVE domain containing protein 12 antibody
    • Zinc finger FYVE domain-containing protein 12 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for RUFY1 using ab230806 at 5 µg/ml in immunohistochemical analysis.

  • Anti-RUFY1 antibody (ab230806) at 0.05 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer) at 35 µg

    Predicted band size: 80 kDa

References

ab230806 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230806.
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