Key features and details
- Goat polyclonal to RUFY1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RUFY1 antibody
See all RUFY1 primary antibodies
DescriptionGoat polyclonal to RUFY1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human small intestine tissue. WB: HeLa cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 80 kDa.|
FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Tissue specificityBroadly expressed, with highest levels in lung, testis, kidney and brain.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RUN domain.
DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
modificationsPhosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- 3000002E04Rik antibody
- FLJ22251 antibody
- FYVE finger protein EIP1 antibody
Formalin-fixed, paraffin-embedded human small intestine tissue stained for RUFY1 using ab230806 at 5 µg/ml in immunohistochemical analysis.
Anti-RUFY1 antibody (ab230806) at 0.05 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 80 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230806 has not yet been referenced specifically in any publications.