Product nameAnti-RUFY1 antibody
See all RUFY1 primary antibodies
DescriptionRabbit polyclonal to RUFY1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RUFY1 aa 658-708. The exact sequence is proprietary.
Database link: Q96T51
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: RUFY1 IP in HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Tissue specificityBroadly expressed, with highest levels in lung, testis, kidney and brain.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RUN domain.
DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
modificationsPhosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Early endosome membrane.
- Information by UniProt
- 3000002E04Rik antibody
- FLJ22251 antibody
- FYVE finger protein EIP1 antibody
RUFY1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241080 at 6 µg/mL per reaction. Western blot was perforemd on the immunoprecipitate using ab241080 at 0.4 µg/mL.
Lane 1: ab241080 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-RUFY1 antibody (ab241080) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
ab241080 has not yet been referenced specifically in any publications.