Overview

  • Product name

  • Description

    Rabbit polyclonal to RUFY1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RUFY1 aa 275-325. The exact sequence is proprietary.
    Database link: Q96T51

  • Positive control

    • IP: RUFY1 IP in HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
    • Tissue specificity

      Broadly expressed, with highest levels in lung, testis, kidney and brain.
    • Sequence similarities

      Contains 1 FYVE-type zinc finger.
      Contains 1 RUN domain.
    • Domain

      The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • Post-translational
      modifications

      Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3000002E04Rik antibody
      • FLJ22251 antibody
      • FYVE finger protein EIP1 antibody
      • FYVE-finger protein EIP1 antibody
      • La binding protein 1 antibody
      • La-binding protein 1 antibody
      • OTTHUMP00000161521 antibody
      • Rab4 interacting protein antibody
      • Rab4-interacting protein antibody
      • RABIP4 antibody
      • RUFY1 antibody
      • RUFY1_HUMAN antibody
      • RUN and FYVE domain containing 1 antibody
      • RUN and FYVE domain-containing protein 1 antibody
      • RUN and FYVE domains containing protein 1 antibody
      • ZFYVE12 antibody
      • Zinc finger FYVE domain containing protein 12 antibody
      • Zinc finger FYVE domain-containing protein 12 antibody
      see all

    Images

    • HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.


      Lane 1: ab241094 used for IP at 6 µg per reaction.
      Lane 2: Control IgG.
      Chemiluminescence detection: 30 seconds.

      For WB a different anti-RUFY1 antibody was used at 1 µg/ml.

    References

    ab241094 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab241094.
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