Key features and details
- Rabbit polyclonal to RUFY2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RUFY2 antibody
See all RUFY2 primary antibodies
DescriptionRabbit polyclonal to RUFY2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human RUFY2 aa 396-655.
LIGLRQQLEEVKAINIEMYQKLQGSEDGLKEKNEIIARLEEKTNKITAAM RQLEQRLQQAEKAQMEAEDEDEKYLQECLSKSDSLQKQISQKEKQLVQLE TDLKIEKEWRQTLQEDLQKEKDALSHLRNETQQIISLKKEFLNLQDENQQ LKKIYHEQEQALQELGNKLSESKLKIEDIKEANKALQGLVWLKDKEATHC KLCEKEFSLSKRKHHCRNCGEIFCNACSDNELPLPSSPKPVRVCDSCHAL LIQRCSSNLP
Database link: Q8WXA3-1
- WB: PC-3 whole cell lysate. IHC-P: Human testis and adrenal gland tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 75, 55, 49 kDa (predicted molecular weight: 76, 71, 75, 47, 44 kDa).|
|IHC-P||1/20 - 1/200.|
RelevanceRUFY2 contains a N-terminal RUN domain, 2 central coiled-coil domains, and a C-terminal FYVE domain containing 2 zinc-binding sites and an SH3 domain recognition motif hence its name RUN and FYVE domain containing protein 2. RUFY2 is expressed in brain, lung, and testis only. RUFY2 reduced secretion of beta-amyloid from human embryonic kidney cells stably expressing APP. The RUFY2 gene maps to a region of chromosome 10q that has been linked to both late-onset Alzheimer disease and elevated plasma levels of pathogenic beta-amyloid. There are 2 isoforms produced by alternative splicing.
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Anti-RUFY2 antibody (ab229924) at 1/1000 dilution + PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 76, 71, 75, 47, 44 kDa
Paraffin-embedded human testis tissue stained for RUFY2 with ab229924 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for RUFY2 with ab229924 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229924 has not yet been referenced specifically in any publications.