Recombinant
RabMAb

Recombinant Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)

Overview

  • Product name

    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098]
    See all RUNX1 / AML1+RUNX3+RUNX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3098] to RUNX1 / AML1 + RUNX3 + RUNX2
  • Host species

    Rabbit
  • Specificity

    The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RUNX1/ AML1 aa 150-250. The exact sequence is proprietary.
    Database link: Q01196

  • Positive control

    • Molt 4 cell lysate, Human fetal thymus tissue lysate, Mouse thymus tissue lysate, Human tonsil tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    Images

    • RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.

      RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.

    • All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261) at 1/1000 dilution

      Lane 1 : Molt 4 cell lysate
      Lane 2 : Human fetal thymus lysate
      Lane 3 : Mouse thymus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 48 kDa

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling RUNX1 / AML1 with ab133261 at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab133261 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133261 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133261 showing -ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133261 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133261 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Tissue sections (3 days embryos)
    Specification
    3 days embryos
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton 0,2%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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    Verified customer

    Submitted Dec 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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