Recombinant Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2
- Suitable for: WB, IP, IHC-P, Flow Cyt, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]
See all RUNX1 / AML1+RUNX3+RUNX2 primary antibodies -
Description
Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-Fr HumanIHC-P HumanIP HumanWB MouseRatHuman -
Immunogen
Synthetic peptide within Human RUNX1/ AML1 (C terminal). The exact sequence is proprietary.
(Peptide available asab177141) -
Positive control
- WB: MOLT4, WEHI-3, CTLL-2 and Raw264.7 cell lysate; mouse and rat thymus tissue lysate, mouse spleen tissue lysate and fetal thymus tissue lysate. IHC: Human tonsil tissue. IP: Molt-4 cell lysate IHC-Fr: Human tonsil tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3099 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199221)
- Alexa Fluor® 647 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199493)
- Alexa Fluor® 594 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207251)
- Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
- Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
|
IHC-Fr |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Mouse
Rat
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).
|
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IP |
1/20.
|
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
|
Flow Cyt |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr |
1/500.
|
Notes |
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WB
1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141). |
IP
1/20. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
Flow Cyt
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-Fr
1/500. |
Target
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Cellular localization
RUNX1 / AML1: Nucleus. RUNX3: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. RUNX2: Nucleus. -
Database links
- Entrez Gene: 860 Human
- Entrez Gene: 861 Human
- Entrez Gene: 864 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 12394 Mouse
- Entrez Gene: 12399 Mouse
- Entrez Gene: 156726 Rat
- Entrez Gene: 367218 Rat
see all
Images
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Molt-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : WEHI-3 (Mouse leukemia lymphoblast) whole cell lysate
Lane 4 : Mouse thymus lysate
Lane 5 : CTLL-2 (Mouse T lymphocyte) whole cell lysate
Lane 6 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 49 kDaBlocking/Diluting buffer and concentration: 5% NFDM /TBST
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ab92336 (purified) at 1/20 immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in 10 μg Molt-4 (Human lymphoblastic leukemia T lymphoblast)whole cell lysate (Lanes 1 and 2, observed at 49 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab92336 in Molt-4 whole cell lysate. For western blotting, ab92336 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg
Predicted band size: 49 kDaBlocking/Diluting buffer and concentration: 5% NFDM /TBST
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RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.
RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1/10000 dilution
Lane 1 : MOLT4
cell lysate
Lane 2 : fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa -
Immunohistochemistry (Frozen sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)
IHC image of RUNX1 / AML1 + RUNX3 + RUNX2 staining in a section of frozen normal human tonsil performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab92336, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)
Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab92336 has been referenced in 44 publications.
- Zhu W et al. CD41-deficient exosomes from non-traumatic femoral head necrosis tissues impair osteogenic differentiation and migration of mesenchymal stem cells. Cell Death Dis 11:293 (2020). PubMed: 32341357
- Ji F et al. The Circular RNA circRNA124534 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Modulation of the miR-496/ß-Catenin Pathway. Front Cell Dev Biol 8:230 (2020). PubMed: 32318572
- Jiang N et al. HIF-1?-regulated miR-1275 maintains stem cell-like phenotypes and promotes the progression of LUAD by simultaneously activating Wnt/ß-catenin and Notch signaling. Theranostics 10:2553-2570 (2020). PubMed: 32194819
- Zhong Y et al. Activation of Steroidogenesis, Anti-Apoptotic Activity, and Proliferation in Porcine Granulosa Cells by RUNX1 Is Negatively Regulated by H3K27me3 Transcriptional Repression. Genes (Basel) 11:N/A (2020). PubMed: 32365901
- Shi H et al. BCL11A Is Oncogenic and Predicts Poor Outcomes in Natural Killer/T-Cell Lymphoma. Front Pharmacol 11:820 (2020). PubMed: 32625084