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  1. Link

    runx1--aml1-antibody-ab23980.pdf

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Anti-RUNX1 / AML1 antibody (ab23980)

  • Datasheet
Reviews (14)Q&A (9)References (126)

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Western blot - Anti-RUNX1 / AML1 antibody (ab23980)
  • Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab23980)

Key features and details

  • Rabbit polyclonal to RUNX1 / AML1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-RUNX1 / AML1 antibody
    See all RUNX1 / AML1 primary antibodies
  • Description

    Rabbit polyclonal to RUNX1 / AML1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human RUNX1/ AML1 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24287)

  • General notes

    Antibody batches of a concentration <1mg/ml will have BSA added to them.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Hemangioblast
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development
    • Neuroscience
    • Development

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RUNX1 / AML1 protein (ab134873)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
  • Tissue specificity

    Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
  • Involvement in disease

    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
    Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
    Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
    Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
    Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.
  • Sequence similarities

    Contains 1 Runt domain.
  • Domain

    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • Post-translational
    modifications

    Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
    Methylated.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q01196 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 861 Human
    • Entrez Gene: 50662 Rat
    • Omim: 151385 Human
    • SwissProt: Q01196 Human
    • SwissProt: Q63046 Rat
    • Unigene: 149261 Human
    • Unigene: 612648 Human
    • Unigene: 11201 Rat
    • Alternative names

      • Acute myeloid leukemia 1 antibody
      • Acute myeloid leukemia 1 protein antibody
      • alpha subunit core binding factor antibody
      • AML 1 antibody
      • AML1 antibody
      • AML1 EVI 1 antibody
      • AML1 EVI 1 fusion protein antibody
      • Aml1 oncogene antibody
      • AMLCR 1 antibody
      • AMLCR1 antibody
      • CBF alpha 2 antibody
      • CBF-alpha-2 antibody
      • CBFA 2 antibody
      • CBFA2 antibody
      • Core binding factor alpha 2 subunit antibody
      • Core binding factor runt domain alpha subunit 2 antibody
      • Core-binding factor subunit alpha-2 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • HGNC antibody
      • Oncogene AML 1 antibody
      • Oncogene AML-1 antibody
      • OTTHUMP00000108696 antibody
      • OTTHUMP00000108697 antibody
      • OTTHUMP00000108699 antibody
      • OTTHUMP00000108700 antibody
      • OTTHUMP00000108702 antibody
      • PEA2 alpha B antibody
      • PEA2-alpha B antibody
      • PEBP2 alpha B antibody
      • PEBP2-alpha B antibody
      • PEBP2A2 antibody
      • PEBP2aB antibody
      • Polyomavirus enhancer binding protein 2 alpha B subunit antibody
      • Polyomavirus enhancer-binding protein 2 alpha B subunit antibody
      • Run1 antibody
      • Runt related transcription factor 1 antibody
      • Runt-related transcription factor 1 antibody
      • RUNX 1 antibody
      • Runx1 antibody
      • RUNX1_HUMAN antibody
      • SL3 3 enhancer factor 1 alpha B subunit antibody
      • SL3-3 enhancer factor 1 alpha B subunit antibody
      • SL3/AKV core binding factor alpha B subunit antibody
      • SL3/AKV core-binding factor alpha B subunit antibody
      see all

    Images

    • Western blot - Anti-RUNX1 / AML1 antibody (ab23980)
      Western blot - Anti-RUNX1 / AML1 antibody (ab23980)
      Anti-RUNX1 / AML1 antibody (ab23980) at 1 µg/ml + Jurkat nuclear extract lysate (ab14844) at 20 µg

      Secondary
      Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48,52,55 kDa why is the actual band size different from the predicted?



      This antibody recognized three distinct bands of between 48 and 55 kDa in Jurkat nuclear lysate. These may represent distinct isoforms of Runx1 or may represent post-translationally modified forms.
    • Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab23980)
      Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab23980)Image courtesy of an anonymous Abreview.
      ab23980 staining RUNX1 / AML1 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (126)

    Publishing research using ab23980? Please let us know so that we can cite the reference in this datasheet.

    ab23980 has been referenced in 126 publications.

    • Kellaway SG  et al. Different mutant RUNX1 oncoproteins program alternate haematopoietic differentiation trajectories. Life Sci Alliance 4:N/A (2021). PubMed: 33397648
    • Li M  et al. Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis. Nat Commun 12:420 (2021). PubMed: 33462242
    • Tang CY  et al. Runx1 is a central regulator of osteogenesis for bone homeostasis by orchestrating BMP and WNT signaling pathways. PLoS Genet 17:e1009233 (2021). PubMed: 33476325
    • Malik N  et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. PLoS Genet 17:e1009553 (2021). PubMed: 33945523
    • Ren S  et al. MiR-18a Aggravates Intracranial Hemorrhage by Regulating RUNX1-Occludin/ZO-1 Axis to Increase BBB Permeability. J Stroke Cerebrovasc Dis 30:105878 (2021). PubMed: 34077824
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

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    1-10 of 14 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse hind limb)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate ph=6
    Permeabilization
    No
    Specification
    mouse hind limb
    Blocking step
    CAS block (commercial) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Neta Felsenthal

    Verified customer

    Submitted Nov 28 2019

    ChIP abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (epithelial)
    Specification
    epithelial
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 08 2019

    Western blot abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human BCP-ALL cell lines)
    Gel Running Conditions
    Reduced Denaturing (7,5% gel)
    Loading amount
    500000 cells
    Specification
    Human BCP-ALL cell lines
    Blocking step
    2% non-fat milk, 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 09 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2017

    Western blot abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Thymus)
    Gel Running Conditions
    Reduced Denaturing (gel 12%)
    Loading amount
    10 µg
    Specification
    Thymus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 12 2016

    Western blot abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (macrophage)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    macrophage
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 22 2014

    Western blot abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (macrophags)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    1e+006 cells
    Specification
    macrophags
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 09 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (glioma cell line C6)
    Specification
    glioma cell line C6
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X 100
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 31 2011

    Western blot abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (bladder, lung, liver, thymus)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    bladder, lung, liver, thymus
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Megan Crow

    Verified customer

    Submitted Jul 19 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RUNX1 / AML1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human glioblastoma cell line)
    Specification
    human glioblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0,1% Triton X-100
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 16 2011

    1-10 of 14 Abreviews

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