Recombinant
RabMAb

Recombinant Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)

Submit a review Submit a question

Overview

  • Product name

    Anti-RUNX1 / AML1 antibody [EPR23044-100]
    See all RUNX1 / AML1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23044-100] to RUNX1 / AML1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human RUNX1/ AML1 aa 200-300. The exact sequence is proprietary.
    Database link: Q01196

  • Positive control

    • WB: THP-1, Jurkat and MOLT-4 lysates. IHC-P: Human breast carcinoma and Human breast tissues. ICC/IF: Jurkat cells. FC: Jurkat cells. IP: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab240639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 48 kDa.

 

 
IP 1/30.

 

 
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

 

 
ICC/IF 1/100.

 

 
Flow Cyt 1/500.

 

 
  • Application notes
    Is unsuitable for ChIP.

    • Target

    • Function

      CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

    • Tissue specificity

      Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

    • Involvement in disease

      Note=A chromosomal aberration involving RUNX1/AML1 is a cause of M2 type acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1T1.
      Note=A chromosomal aberration involving RUNX1/AML1 is a cause of therapy-related myelodysplastic syndrome (T-MDS). Translocation t(3;21)(q26;q22) with EAP or MECOM.
      Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelogenous leukemia (CML). Translocation t(3;21)(q26;q22) with EAP or MECOM.
      Note=A chromosomal aberration involving RUNX1/AML1 is found in childhood acute lymphoblastic leukemia (ALL). Translocation t(12;21)(p13;q22) with TEL. The translocation fuses the 3'-end of TEL to the alternate 5'-exon of AML-1H.
      Note=A chromosomal aberration involving RUNX1 is found in acute leukemia. Translocation t(11,21)(q13;q22) that forms a MACROD1-RUNX1 fusion protein.
      Defects in RUNX1 are the cause of familial platelet disorder with associated myeloid malignancy (FPDMM) [MIM:601399]. FPDMM is an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
      Note=A chromosomal aberration involving RUNX1/AML1 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
      Note=A chromosomal aberration involving RUNX1/AML1 is a cause of chronic myelomonocytic leukemia. Inversion inv(21)(q21;q22) with USP16.

    • Sequence similarities

      Contains 1 Runt domain.

    • Domain

      A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

    • Post-translational
      modifications

      Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with MYST3.
      Methylated.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Acute myeloid leukemia 1 antibody
        • Acute myeloid leukemia 1 protein antibody
        • alpha subunit core binding factor antibody
        • AML 1 antibody
        • AML1 antibody
        • AML1 EVI 1 antibody
        • AML1 EVI 1 fusion protein antibody
        • Aml1 oncogene antibody
        • AMLCR 1 antibody
        • AMLCR1 antibody
        • CBF alpha 2 antibody
        • CBF-alpha-2 antibody
        • CBFA 2 antibody
        • CBFA2 antibody
        • Core binding factor alpha 2 subunit antibody
        • Core binding factor runt domain alpha subunit 2 antibody
        • Core-binding factor subunit alpha-2 antibody
        • EVI 1 antibody
        • EVI1 antibody
        • HGNC antibody
        • Oncogene AML 1 antibody
        • Oncogene AML-1 antibody
        • OTTHUMP00000108696 antibody
        • OTTHUMP00000108697 antibody
        • OTTHUMP00000108699 antibody
        • OTTHUMP00000108700 antibody
        • OTTHUMP00000108702 antibody
        • PEA2 alpha B antibody
        • PEA2-alpha B antibody
        • PEBP2 alpha B antibody
        • PEBP2-alpha B antibody
        • PEBP2A2 antibody
        • PEBP2aB antibody
        • Polyomavirus enhancer binding protein 2 alpha B subunit antibody
        • Polyomavirus enhancer-binding protein 2 alpha B subunit antibody
        • Run1 antibody
        • Runt related transcription factor 1 antibody
        • Runt-related transcription factor 1 antibody
        • RUNX 1 antibody
        • Runx1 antibody
        • RUNX1_HUMAN antibody
        • SL3 3 enhancer factor 1 alpha B subunit antibody
        • SL3-3 enhancer factor 1 alpha B subunit antibody
        • SL3/AKV core binding factor alpha B subunit antibody
        • SL3/AKV core-binding factor alpha B subunit antibody
        see all

      Images

      • Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        All lanes : Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639) at 1/1000 dilution

        Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
        Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
        Lane 3 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 48 kDa
        Observed band size: 27-55 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 23352661, 29296779).  The RUNX1 gene has several isoforms, 3 major isotypes. RUNX1b is broadly expressed, and RUNX1a overexpression has been reported in AML. 

        Exposure time: Lane 1: 48 seconds Lanes 2-3: 26 seconds

      • Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Immunoprecipitation - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        RUNX1 / AML1 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug with ab240639 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240639 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

        Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug

        Lane 2: ab240639 IP in Jurkat whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240639 in Jurkat whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 10 seconds

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)

        Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling RUNX1 / AML1 with ab240639 at 1/2000 dilution (0.25 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast tissue is observed. The section was incubated with ab240639 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)

        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RUNX1 / AML1 with ab240639 at 1/2000 dilution (0.25 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast carcinoma (PMID: 24967588) tissue is observed. The section was incubated with ab240639 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling RUNX1 / AML1 with ab240639 at 1/100 dilution, followed by ab240639 anti-RUNX1/AML1 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in Jurkat cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab240639 anti-RUNX1/AML1 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      • Flow Cytometry - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)
        Flow Cytometry - Anti-RUNX1 / AML1 antibody [EPR23044-100] (ab240639)

        Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling RUNX1 / AML1 with ab240639 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      References

      ab240639 has not yet been referenced specifically in any publications.

      Publishing research using ab240639? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab240639.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com