Application
Western blot
Sample
Human Cell lysate - whole cell (AML Cells Kasumi)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
AML Cells Kasumi
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Concentration
1 µg/ml
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~50 kDa
Additional data
Additional Notes
Kasumi cells contain Runx and a RUNX fusion (AML-ETO). This antibody only detects RUNX adequately. The AML-ETO antibody from Abcam detected the fusion.
MR. Minaam Abbas
Verified customer
Submitted Jul 13 2021