Anti-RUNX1T1/ETO/CDR antibody (ab124269)
Key features and details
- Rabbit polyclonal to RUNX1T1/ETO/CDR
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RUNX1T1/ETO/CDR antibody
See all RUNX1T1/ETO/CDR primary antibodies -
Description
Rabbit polyclonal to RUNX1T1/ETO/CDR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human RUNX1T1/ETO/CDR aa 554-604.
Database link: NP_001185555.1 -
Positive control
- 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab124269 was affinity purified using an epitope specific to RUNX1T1/ETO/CDR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
|
|
IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/500 - 1/2000. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. -
Tissue specificity
Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary. -
Involvement in disease
Note=A chromosomal aberration involving RUNX1T1 is a cause of acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1.
Defects in RUNX1T1 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain. -
Domain
The TAFH domain mediates interaction with transcription regulators. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 862 Human
- Entrez Gene: 12395 Mouse
- Entrez Gene: 362489 Rat
- Omim: 133435 Human
- SwissProt: Q06455 Human
- SwissProt: Q61909 Mouse
- Unigene: 368431 Human
- Unigene: 470961 Mouse
see all -
Alternative names
- Acute myelogenous leukemia 1 translocation 1 cyclin D related antibody
- Acute myelogenous leukemia 1 translocation 1 protein antibody
- AML1 MTG8 antibody
see all
Images
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All lanes : Anti-RUNX1T1/ETO/CDR antibody (ab124269) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds -
ab124269 at 1 µg/ml staining RUNX1T1/ETO/CDR in 293T whole cell lysate immunoprecipitated using ab124269 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 68 kDa. Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124269 has been referenced in 3 publications.
- Mu G & Chen F Oncogenic Roles Of A Histone Methyltransferase SETDB2 In AML1-ETO Positive AML. Cancer Manag Res 12:783-792 (2020). PubMed: 32099474
- Liu S et al. RUNX1 inhibits proliferation and induces apoptosis of t(8;21) leukemia cells via KLF4 mediated transactivation of P57. Haematologica N/A:N/A (2019). PubMed: 30792202
- Zaidi SK et al. An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells. Oncotarget 8:39994-40005 (2017). PubMed: 28611288