Key features and details
- Rabbit polyclonal to RUNX1T1/ETO/CDR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RUNX1T1/ETO/CDR antibody
See all RUNX1T1/ETO/CDR primary antibodies
DescriptionRabbit polyclonal to RUNX1T1/ETO/CDR
Specificityab231787 was raised against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RUNX1T1/ETO/CDR conjugated to keyhole limpet haemocyanin. (Three different KLH-conjugated synthetic peptides from the AML-ETO (RUNX1) fusion protein).
Database link: Q06455
- WB: KAS-6/1 and SKNO-1 nuclear extracts.
This product was previously labelled as RUNX1T1 / ETO
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
Our Abpromise guarantee covers the use of ab231787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 68 kDa.|
FunctionTranscription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12.
Tissue specificityMost abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.
Involvement in diseaseNote=A chromosomal aberration involving RUNX1T1 is a cause of acute myeloid leukemia (AML-M2). Translocation t(8;21)(q22;q22) with RUNX1/AML1.
Defects in RUNX1T1 may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similaritiesBelongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain.
DomainThe TAFH domain mediates interaction with transcription regulators.
- Information by UniProt
- Acute myelogenous leukemia 1 translocation 1 cyclin D related antibody
- Acute myelogenous leukemia 1 translocation 1 protein antibody
- AML1 MTG8 antibody
All lanes : Anti-RUNX1T1/ETO/CDR antibody (ab231787) at 1/1000 dilution
Lane 1 : Kasumi-1 (human acute myeloblastic leukemia) nuclear extracts
Lane 2 : SKNO-1 (human acute myeloblastic leukaemia) nuclear extracts
Predicted band size: 68 kDa
Dilution buffer: TBS-Tween containing 5% skimmed milk.
Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with ab231787 diluted 1/1,000 in TBS-Tween containing 5% skimmed milk.
ab231787 recognizes the ETO part (lane 2) of the fusion protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231787 has not yet been referenced specifically in any publications.