Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab23981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19092814
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).

Abcam recommends using 3% milk as the blocking agent.

EMSA Use at an assay dependent concentration.

Target

  • Function

    Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation.
  • Tissue specificity

    Specifically expressed in osteoblasts.
  • Involvement in disease

    Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.
  • Sequence similarities

    Contains 1 Runt domain.
  • Domain

    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites.
  • Post-translational
    modifications

    Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acute myeloid leukemia 3 protein antibody
    • Alpha subunit 1 antibody
    • AML3 antibody
    • CBF alpha 1 antibody
    • CBF-alpha-1 antibody
    • CBFA1 antibody
    • CCD antibody
    • CCD1 antibody
    • Cleidocranial dysplasia 1 antibody
    • Core binding factor antibody
    • Core binding factor runt domain alpha subunit 1 antibody
    • Core binding factor subunit alpha 1 antibody
    • Core-binding factor subunit alpha-1 antibody
    • MGC120022 antibody
    • MGC120023 antibody
    • Oncogene AML 3 antibody
    • Oncogene AML-3 antibody
    • OSF 2 antibody
    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific transcription factor 2 antibody
    • Osteoblast-specific transcription factor 2 antibody
    • OTTHUMP00000016533 antibody
    • PEA2 alpha A antibody
    • PEA2-alpha A antibody
    • PEA2aA antibody
    • PEBP2 alpha A antibody
    • PEBP2-alpha A antibody
    • PEBP2A1 antibody
    • PEBP2A2 antibody
    • PEBP2aA antibody
    • PEBP2aA1 antibody
    • Polyomavirus enhancer binding protein 2 alpha A subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha A subunit antibody
    • Runt domain antibody
    • Runt related transcription factor 2 antibody
    • Runt-related transcription factor 2 antibody
    • RUNX2 antibody
    • RUNX2_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha A subunit antibody
    • SL3-3 enhancer factor 1 alpha A subunit antibody
    • SL3/AKV core binding factor alpha A subunit antibody
    • SL3/AKV core-binding factor alpha A subunit antibody
    see all

Images

  • ab23981 at a 1/1000 dilution staining RUNX2 in human U2-OS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked using 2% serum. The secondary used was an Alexa Fluor® 488 conjugated chicken anti-mouse at a 1/1000 dilution.

    See Abreview

  • All lanes : Anti-RUNX2 antibody (ab23981) at 1/1000 dilution

    Lane 1 : Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks.
    Lane 2 : Bone tissue (tibia) from 20 weeks old Wistar female rats.
    Lane 3 : Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 40 seconds

    See Abreview

  • Anti-RUNX2 antibody (ab23981) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Nuclear Extract at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa


    Exposure time: 8 minutes

References

This product has been referenced in:

  • Aquino-Martínez R  et al. Calcium mimics the chemotactic effect of conditioned media and is an effective inducer of bone regeneration. PLoS One 14:e0210301 (2019). Read more (PubMed: 30608979) »
  • Zhuang L  et al. miR-205 targets runt-related transcription factor 2 to inhibit human pancreatic cancer progression. Oncol Lett 17:843-848 (2019). Read more (PubMed: 30655837) »
See all 106 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (connective tissue around bone graft in maxillae)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution
Permeabilization
Yes - 0.5% Triton
Specification
connective tissue around bone graft in maxillae
Blocking step
R.T.U. Animal-Free Block and Diluent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Formaldehyde

Dr. Jinlong Chen

Verified customer

Submitted Sep 20 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (maxillary edentulous zone-periosteum)
Permeabilization
Yes - 0.5% Triton
Specification
maxillary edentulous zone-periosteum
Blocking step
R.T.U. Animal-Free Block and Diluent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Formaldehyde

Dr. Jinlong Chen

Verified customer

Submitted Sep 20 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MSC)
Specification
MSC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Tina Yuan

Verified customer

Submitted Sep 17 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Permeabilization
No
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
Permeabilization
Yes - 0.05% triton-x100
Specification
bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 27 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vetor Unmasking solution
Permeabilization
Yes - 0.05% triton-x100
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 27 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
30 µg
Treatment
transfecting with human Runx2 plasmid
Specification
HEK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jul 02 2019

Application
Western blot
Sample
Chicken Cell lysate - whole cell (DF-1)
Gel Running Conditions
Reduced Denaturing (12% SDS Gel)
Loading amount
20 µg
Specification
DF-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Spenser Smith

Verified customer

Submitted May 16 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
Yes - 0.025% Triton x-100
Specification
Bone tissue (Femur)
Blocking step
Ab93695 ABC kit as blocking agent as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 08 2012

1-10 of 16 Abreviews or Q&A

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