• Product name
    Anti-RUNX3 antibody - ChIP Grade
    See all RUNX3 primary antibodies
  • Description
    Rabbit polyclonal to RUNX3 - ChIP Grade
  • Host species
  • Tested applications
    Suitable for: ChIP, WB, EMSA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Raji nuclear extract. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431



Our Abpromise guarantee covers the use of ab11905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 20714105
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
EMSA Use at an assay dependent concentration.
ICC/IF 1/200.


  • Function
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
  • Sequence similarities
    Contains 1 Runt domain.
  • Domain
    A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • Post-translational
    Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
  • Cellular localization
    Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acute myeloid leukemia 2 protein antibody
    • Acute myeloid leukemia gene 2 antibody
    • AML 2 antibody
    • AML2 antibody
    • CBF alpha 3 antibody
    • CBF-alpha-3 antibody
    • CBFA 3 antibody
    • CBFA3 antibody
    • Core binding factor alpha 3 subunit antibody
    • core binding factor antibody
    • Core binding factor runt domain alpha subunit 3 antibody
    • Core binding factor subunit alpha 3 antibody
    • core-binding factor antibody
    • Core-binding factor subunit alpha-3 antibody
    • Oncogene AML 2 antibody
    • Oncogene AML-2 antibody
    • PEA2 alpha C antibody
    • PEA2-alpha C antibody
    • PEBP2 alpha C antibody
    • PEBP2-alpha C antibody
    • Pebp2a3 antibody
    • PEBP2aC antibody
    • Polyomavirus enhancer binding protein 2 alpha C subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha C subunit antibody
    • runt domain alpha subunit 3 antibody
    • runt related transcription factor 3 antibody
    • Runt-related transcription factor 3 antibody
    • RUNX 3 antibody
    • Runx3 antibody
    • RUNX3_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha C subunit antibody
    • SL3-3 enhancer factor 1 alpha C subunit antibody
    • SL3/AKV core binding factor alpha C subunit antibody
    • SL3/AKV core-binding factor alpha C subunit antibody
    • Transcription factor AML2 antibody
    see all


  • Detection of AML2 by EMSA analysis. Nushift polyclonal antibody against AML2 factor creates a supershift in EMSA (lane 4).
  • ICC/IF image of ab11905 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11905 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Morita K  et al. RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice. Blood Adv 2:509-515 (2018). Read more (PubMed: 29500219) »
  • Zheng J  et al. DNA methylation affects metastasis of renal cancer and is associated with TGF-ß/RUNX3 inhibition. Cancer Cell Int 18:56 (2018). Read more (PubMed: 29651226) »

See all 9 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (RAJI cell lysate and HEK293T oveexpressing RUNX3)
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Loading amount
35 µg
RUNX3 plasmid ytransfection for overexpresssion in 293T cells
RAJI cell lysate and HEK293T oveexpressing RUNX3
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Yupen Chung

Verified customer

Submitted Apr 25 2016

Thank you for your patience with this inquiry. I am very sorry for the delay.
We sourced this particular product externally and regrettably the laboratory cannot provide the information, which isoform of the RUNX3 was used to produce this antibody....

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Thank you for your phone call.

Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab11905 in mouse. This code will give you xxx off your next order before ...

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Thank you for taking the time to submit these comments. It looks to me from the gel that the gel has not run out properly. Could you please enquire with the customer as to how the gel was made and how long the running gel and stacking gel were left to ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab11905. For Western blotting, we do suggest using this antibody at 1:500 - 1:1000 and so I do suggest trying ab11905 at 1:500 with incubation overnight at 4C. W...

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