Key features and details
- Rabbit polyclonal to RWDD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RWDD1 antibody
See all RWDD1 primary antibodies
DescriptionRabbit polyclonal to RWDD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RWDD1 aa 193-243. The exact sequence is proprietary.
Database link: Q9H446
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 µl/mg lysate
FunctionProtects DRG2 from proteolytic degradation.
Sequence similaritiesBelongs to the RWDD1/GIR2 family.
Contains 1 RWD domain.
- Information by UniProt
- CGI 24 antibody
- DFRP2 antibody
- DRG family regulatory protein 2 antibody
RWDD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241191 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab241191 at 1/1000 dilution.
Lane 1: ab241191 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-RWDD1 antibody (ab241191) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241191 has not yet been referenced specifically in any publications.