Recombinant Anti-RWDD1 antibody [EPR13715(B)] (ab181994)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13715(B)] to RWDD1
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RWDD1 antibody [EPR13715(B)]
See all RWDD1 primary antibodies -
Description
Rabbit monoclonal [EPR13715(B)] to RWDD1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells; Jurkat, HeLa, 293, A673 cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13715(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
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IP |
1/60.
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Notes |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
IP
1/60. |
Target
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Function
Protects DRG2 from proteolytic degradation. -
Sequence similarities
Belongs to the RWDD1/GIR2 family.
Contains 1 RWD domain. - Information by UniProt
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Database links
- Entrez Gene: 51389 Human
- Entrez Gene: 66521 Mouse
- Entrez Gene: 259218 Rat
- GenBank: NM_015952 Human
- SwissProt: Q9H446 Human
- SwissProt: Q9CQK7 Mouse
- SwissProt: Q99ND9 Rat
- Unigene: 532164 Human
see all -
Alternative names
- CGI 24 antibody
- DFRP2 antibody
- DRG family regulatory protein 2 antibody
see all
Images
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All lanes : Anti-RWDD1 antibody [EPR13715(B)] (ab181994) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A673 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHelacells labeling RWDD1with ab181994at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilutionwas used as a secondary antibody.
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Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181994 has not yet been referenced specifically in any publications.