Key features and details
- Rabbit monoclonal [EPR13715(B)] to RWDD1
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-RWDD1 antibody [EPR13715(B)]
See all RWDD1 primary antibodies
DescriptionRabbit monoclonal [EPR13715(B)] to RWDD1
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RWDD1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H446
- HeLa cells; Jurkat, HeLa, 293, A673 cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181994 in the following tested applications.
|WB||1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProtects DRG2 from proteolytic degradation.
Sequence similaritiesBelongs to the RWDD1/GIR2 family.
Contains 1 RWD domain.
- Information by UniProt
- CGI 24 antibody
- DFRP2 antibody
- DRG family regulatory protein 2 antibody
All lanes : Anti-RWDD1 antibody [EPR13715(B)] (ab181994) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A673 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling RWDD1 with ab181994 at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilution was used as a secondary antibody.
Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
ab181994 has not yet been referenced specifically in any publications.