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Rabbit polyclonal to RWDD2B
antigen, corresponding to amino acids 252-319 of Human RWDD2B.
- Human skeletal muscle tissue; Transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Contains 1 RWD domain.
Information by UniProt
- C21orf6 antibody
- GL011 antibody
- ORF5 antibody
Immunocytochemistry/ Immunofluorescence - Anti-RWDD2B antibody (ab121567)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121567 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RWDD2B antibody (ab121567)
ab121567 at 1/50 dilution staining RWDD2B in Paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry.
Western blot - Anti-RWDD2B antibody (ab121567)
All lanes : Anti-RWDD2B antibody (ab121567) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : RWDD2B transfected HEK239T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"