Brains were perfused with 4% paraformaldehyde.
Sections cut and stained at 20 microns for immunohistochemistry on slides.
Blocking agent also has 1% fish gelatin.
This antibody produced very little background and no bleed-through. It was a very good nuclear stain.
Abcam user community
Submitted Jan 07 2014
Get resources and offers direct to your inboxSign up