Western blot abreview for Anti-Retinoid X Receptor gamma antibody

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Abreviews
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Schwann cells)
Specification
Schwann cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Bands
Specific: about 60 kDa Non-specific: strong band about 35 kDa
Exposure
40 second(s)
Negative control
siRNA against RXRgamma

Additional data

Additional Notes
Cells lysed directly in Laemmli sample buffer Marker size on image: 250,150,100,75,50,37,25,20

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Submitted Feb 27 2014

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