Immunocytochemistry/ Immunofluorescence abreview for Anti-Retinoid X Receptor gamma antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO K1 cells expressing Human Retinoid X Receptor)
Specification
CHO K1 cells expressing Human Retinoid X Receptor
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS + 3% dried milk + 0.1% Triton X-100

Secondary antibody

Name
Non-Abcam antibody was used: FITC labelled Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/400

Additional data

Additional Notes
CHO K1 cells expressing Human Retinoid X Receptor gamma (RXRg) clearly show nuclear localization of Human RXRg when grown in the presence of F12 + 10% FBS.
The image on the left shows the expression of Human RXRg as detected by anti-RXRg (ab15518) and the image on the right shows the corresponding DAPI nuclear staining.

Dr. Mahesh Mathrubutham

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Submitted Sep 05 2007

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