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    rxrg-antibody-ab53162.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-RXRG antibody (ab53162)

  • Datasheet
Reviews (2) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RXRG antibody (ab53162)

    Key features and details

    • Rabbit polyclonal to RXRG
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human RXRG protein (ab114438)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-RXRG antibody
      See all RXRG primary antibodies
    • Description

      Rabbit polyclonal to RXRG
    • Host species

      Rabbit
    • Specificity

      ab53162 detects endogenous levels of total Retinoic X Receptor gamma protein.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human RXRG.

    • General notes

       This product was previously labelled as Retinoid X Receptor gamma

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

      Without Mg+2 and Ca+2
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab53162 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Retinoic & Retinoid
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Retinoic & Retinoid
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Retanoic and retanoid

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Hep G2 nuclear extract lysate (ab14660)
    • Recombinant Protein

      • Recombinant Human RXRG protein (ab114438)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab53162 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use a concentration of 2 - 4 µg/ml.
    Notes
    IHC-P
    Use a concentration of 2 - 4 µg/ml.

    Target

    • Function

      Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P48443 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6258 Human
      • Omim: 180247 Human
      • SwissProt: P48443 Human
      • Unigene: 26550 Human
      • Alternative names

        • MGC109416 antibody
        • NR2B3 antibody
        • Nuclear receptor subfamily 2 group B member 3 antibody
        • OTTHUMP00000060418 antibody
        • Retanoic X receptor gamma antibody
        • Retinoic acid receptor RXR gamma antibody
        • Retinoic acid receptor RXR-gamma antibody
        • Retinoid X receptor gamma antibody
        • RXR G antibody
        • RXR gamma antibody
        • RXRC antibody
        • Rxrg antibody
        • RXRG_HUMAN antibody
        • RXRgamma antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RXRG antibody (ab53162)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RXRG antibody (ab53162)

        Ab53162 staining RXRG in human nucleus accumbens.
        Left panel: with primary antibody at a dilution of 4µg/ml. Right panel: isotype control.
        Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH9.0 in a Dako PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab53162? Please let us know so that we can cite the reference in this datasheet.

      ab53162 has been referenced in 2 publications.

      • Kovalevich J  et al. Cocaine induces nuclear export and degradation of neuronal retinoid X receptor-? via a TNF-a/JNK- mediated mechanism. J Neuroimmune Pharmacol 10:55-73 (2015). WB, ICC/IF ; Mouse . PubMed: 25586717
      • Donmez G  et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . PubMed: 20655472

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      IHC - Wholemount abreview for Anti-Retinoid X Receptor gamma antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      IHC - Wholemount
      Sample
      Locusta migratoria Embryo (Nervous system)
      Specification
      Nervous system
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 10 2017

      Western blot abreview for Anti-Retinoid X Receptor gamma antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (293T, HepG2, MEF)
      Loading amount
      20 µg
      Specification
      293T, HepG2, MEF
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Mr. Dongil Kim

      Verified customer

      Submitted Jan 21 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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