Key features and details
- Rabbit polyclonal to Ryanodine receptor 2/RYR-2 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ryanodine receptor 2/RYR-2 antibody - N-terminal
See all Ryanodine receptor 2/RYR-2 primary antibodies
DescriptionRabbit polyclonal to Ryanodine receptor 2/RYR-2 - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
Synthetic peptide within Human Ryanodine receptor 2/RYR-2 (N terminal). The exact sequence is proprietary.
Database link: Q92736
Previously labelled as Ryanodine receptor 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab196355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/5000. Predicted molecular weight: 565 kDa.|
FunctionCalcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
Tissue specificityDetected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
Involvement in diseaseFamilial arrhythmogenic right ventricular dysplasia 2
Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
Sequence similaritiesBelongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains.
Developmental stageExpressed in myometrium during pregnancy.
DomainThe calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
modificationsChannel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
Cellular localizationSarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
- Information by UniProt
- ARVC2 antibody
- ARVD2 antibody
- Cardiac muscle ryanodine receptor antibody
ab196355 has been referenced in 1 publication.
- Ruiz-Meana M et al. Ryanodine Receptor Glycation Favors Mitochondrial Damage in the Senescent Heart. Circulation 139:949-964 (2019). PubMed: 30586718