Product nameAnti-Ryanodine receptor 2/RYR-2 (phospho S2808) antibody
See all Ryanodine receptor 2/RYR-2 primary antibodies
DescriptionRabbit polyclonal to Ryanodine receptor 2/RYR-2 (phospho S2808)
ab59225 detects endogenous levels of Ryanodine receptor 2/RYR-2 only when phosphorylated at serine 2808.
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Ryanodine receptor 2/RYR-2. Synthetic phosphopeptide derived from human Ryanodine Receptor around the phosphorylation site of serine 2808 (R-I-SP-Q-T).
Database link: Q92736
- WB: K562 cells treated with IL4. IHC-P: Human kidney and brain tissue.
Previously labelled as Ryanodine receptor 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab59225 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab59225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||Use at an assay dependent concentration. PubMed: 21156754|
FunctionCalcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
Tissue specificityDetected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
Involvement in diseaseFamilial arrhythmogenic right ventricular dysplasia 2
Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy
Sequence similaritiesBelongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains.
Developmental stageExpressed in myometrium during pregnancy.
DomainThe calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.
modificationsChannel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium.
Cellular localizationSarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
- Information by UniProt
- ARVC2 antibody
- ARVD2 antibody
- Cardiac muscle ryanodine receptor antibody
All lanes : Anti-Ryanodine receptor 2/RYR-2 (phospho S2808) antibody (ab59225) at 1/1000 dilution
Lane 1 : K562 untreated with IL4
Lane 2 : K562 treated with IL4 10ng/mL for 5 minutes
Lysates/proteins at 20 µg per lane.
All lanes : Dylight 800 secondary antibody at 1/10000 dilution
Loading control is Actin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ryanodine receptor 2/RYR-2 (phospho S2808) antibody (ab59225) at 1/100 dilution.
ab59225 (1/50 dilution) staining Ryanodine receptor 2/RYR-2 in human brain tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunizing peptide.
ab59225 has been referenced in 14 publications.
- Potes Y et al. Overweight in the Elderly Induces a Switch in Energy Metabolism that Undermines Muscle Integrity. Aging Dis 10:217-230 (2019). PubMed: 31011474
- Jacko D et al. Resistance exercise-induced muscle fatigue is not accompanied by increased phosphorylation of ryanodine receptor 1 at serine 2843. PLoS One 13:e0199307 (2018). PubMed: 29953482
- Pyun JH et al. Cardiac specific PRMT1 ablation causes heart failure through CaMKII dysregulation. Nat Commun 9:5107 (2018). PubMed: 30504773
- Park SJ et al. Specific Sirt1 Activator-mediated Improvement in Glucose Homeostasis Requires Sirt1-Independent Activation of AMPK. EBioMedicine 18:128-138 (2017). WB . PubMed: 28396013
- Hu LR et al. Deregulated Ca(2+) cycling underlies the development of arrhythmia and heart disease due to mutant obscurin. Sci Adv 3:e1603081 (2017). WB, IF ; Mouse . PubMed: 28630914