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Asked on Sep 18 2012
Thank you for contacting Abcam.
I am sorry about the issues you have been having with ab2827 in IF on cultured rat cells. The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on rat samples.
Based on the information and the images that you provided, I do not think that protocol tips would help resolve the issue that you are having and so I would like to offer to either replace or refund the cost of the antibody. A potential replacement antibody would be:
Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody that would be very helpful.
I look forward to your reply.
Answered on Sep 18 2012