Western blot abreview for Anti-Ryanodine Receptor [C3-33] antibody

Good
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Sheep Tissue lysate - whole (cardiac)
Specification
cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% milk in TBST
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-Mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: >250 kDa
Exposure
1 minute(s) and 45 second(s)
Positive control
sheep cardiac tissue lysed in RIPA with protease inhibitors

Additional data

Additional Notes
Hybond LFP PVDF

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Submitted Nov 15 2013

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