Overview

  • Product name

  • Description

    Rabbit polyclonal to RYBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Monkey, Macaque monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human RYBP aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab126636)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2; K562; A549; JEG3. This antibody gave a positive signal in the following cell lines: HeLa, HepG2, MCF-7 (Methanol fixed), and in HepG2 (Formaldehyde fixed).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
  • Tissue specificity

    Widely expressed with highest levels in lymphoid tissues and placenta.
  • Sequence similarities

    Contains 1 RanBP2-type zinc finger.
  • Cellular localization

    Nucleus. Cytoplasm. Primarily found in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2410018J24Rik antibody
    • AAP 1 antibody
    • AAP1 antibody
    • APAP 1 antibody
    • APAP-1 antibody
    • APAP1 antibody
    • Apoptin associating protein 1 antibody
    • Apoptin-associating protein 1 antibody
    • cb337 antibody
    • Death effector domain associated factor antibody
    • Death effector domain-associated factor antibody
    • Death effector domain-associated factor B antibody
    • DED associated factor antibody
    • DED-associated factor antibody
    • DED-associated factor B antibody
    • DEDAF antibody
    • MGC62213 antibody
    • RING1 and YY1 binding protein a antibody
    • RING1 and YY1 binding protein antibody
    • RING1 and YY1-binding protein antibody
    • RING1 and YY1-binding protein B antibody
    • Ring1 interactor RYBP antibody
    • RYBP antibody
    • RYBP_HUMAN antibody
    • rybpa antibody
    • wu:fi34f03 antibody
    • YEAF 1 antibody
    • YEAF1 antibody
    • YY1 and E4TF1 associated factor 1 antibody
    • YY1 and E4TF1-associated factor 1 antibody
    • YY1- and E4TF1/GABP-associated factor 1 antibody
    • zgc:153842 antibody
    see all

Images

  • All lanes : Anti-RYBP antibody (ab107896) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • ICC/IF image of ab107896 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107896 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa and HepG2 cells, and in 4% Formaldehyde fixed (10min) HepG2 cells at 1ug/ml.

References

ab107896 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456789und sollte morgen bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Ich habe Rückmeldung aus dem Labor erhalten: Leider sieht es so aus, dass der ab128104 Anti-RYBP antibody [1B2] nur gegen das rekombinante Protein getestet wurde.

Soll ich Ihnen daher (wie besprochen) den ab107896 Anti-RYBP antibody zukommen lassen?

Read More

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