Anti-RYBP antibody (ab5976)
Key features and details
- Rabbit polyclonal to RYBP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-RYBP antibody
See all RYBP primary antibodies -
Description
Rabbit polyclonal to RYBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
TPKGDMSAVNDESF
, corresponding to amino acids 215-228 of Human DEDAF. -
Positive control
- A549, HepG2 or 3T3 whole cell lysate.
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General notes
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
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If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
DEDAF Antibody is affinity chromatography purified via peptide column. -
Primary antibody notes
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 32 kDa. Can be blocked with DEDAF peptide (human) .
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IHC-P |
Use a concentration of 5 - 10 µg/ml.
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ICC/IF | (1) |
Use a concentration of 1 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 32 kDa. Can be blocked with DEDAF peptide (human) . |
IHC-P
Use a concentration of 5 - 10 µg/ml. |
ICC/IF
Use a concentration of 1 - 20 µg/ml. |
Target
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Function
May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis. -
Tissue specificity
Widely expressed with highest levels in lymphoid tissues and placenta. -
Sequence similarities
Contains 1 RanBP2-type zinc finger. -
Cellular localization
Nucleus. Cytoplasm. Primarily found in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23429 Human
- Entrez Gene: 56353 Mouse
- Omim: 607535 Human
- SwissProt: Q8N488 Human
- SwissProt: Q8CCI5 Mouse
- Unigene: 7910 Human
- Unigene: 321633 Mouse
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Alternative names
- 2410018J24Rik antibody
- AAP 1 antibody
- AAP1 antibody
see all
Images
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Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.µ g /ml. -
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/ml.
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ab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody.
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Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab5976 has been referenced in 10 publications.
- Yew WP et al. Early treatment with minocycline following stroke in rats improves functional recovery and differentially modifies responses of peri-infarct microglia and astrocytes. J Neuroinflammation 16:6 (2019). PubMed: 30626393
- Li X et al. Circular RNA circPVT1 Promotes Proliferation and Invasion Through Sponging miR-125b and Activating E2F2 Signaling in Non-Small Cell Lung Cancer. Cell Physiol Biochem 51:2324-2340 (2018). PubMed: 30537738
- Li H et al. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell Stem Cell 20:462-477.e6 (2017). PubMed: 28111200
- Bian F et al. Polycomb repressive complex 1 controls uterine decidualization. Sci Rep 6:26061 (2016). PubMed: 27181215
- Eid A & Torres-Padilla ME Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis. Epigenetics 11:389-97 (2016). PubMed: 27081692
- Jacinto FV et al. The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev 29:1224-38 (2015). PubMed: 26080816
- He J et al. Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of developmental genes. Nat Cell Biol 15:373-84 (2013). Mouse . PubMed: 23502314
- Toth Z et al. Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection. PLoS Pathog 9:e1003813 (2013). PubMed: 24367262
- Ritter HD et al. The Unliganded Glucocorticoid Receptor Positively Regulates the Tumor Suppressor Gene BRCA1 through GABP Beta. Mol Cancer Res 10:558-69 (2012). ChIP . PubMed: 22328717
- Tavares L et al. RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3. Cell 148:664-78 (2012). IF . PubMed: 22325148