- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-RYBP antibody
See all RYBP primary antibodies -
DescriptionRabbit polyclonal to RYBP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
TPKGDMSAVNDESF
, corresponding to amino acids 215-228 of Human DEDAF. -
Positive control
- A549, HepG2 or 3T3 whole cell lysate.
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General notesStable for one year.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesDEDAF Antibody is affinity chromatography purified via peptide column.
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Primary antibody notesApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab5976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with RYBP peptide (ab6097). Can be blocked with DEDAF peptide (human) . | |
| IHC-P | Use a concentration of 5 - 10 µg/ml. | |
| ICC/IF | Use a concentration of 1 - 20 µg/ml. |
Target
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FunctionMay be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
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Tissue specificityWidely expressed with highest levels in lymphoid tissues and placenta.
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Sequence similaritiesContains 1 RanBP2-type zinc finger.
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Cellular localizationNucleus. Cytoplasm. Primarily found in the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 23429 Human
- Entrez Gene: 56353 Mouse
- Entrez Gene: 312603 Rat
- Omim: 607535 Human
- SwissProt: Q8N488 Human
- SwissProt: Q8CCI5 Mouse
- Unigene: 7910 Human
- Unigene: 321633 Mouse
see all -
Alternative names
- 2410018J24Rik antibody
- AAP 1 antibody
- AAP1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RYBP antibody (ab5976)Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/ml.
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Western blot - Anti-RYBP antibody (ab5976)Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)This image is courtesy of an Abreview submitted by Dr. Ligia Tavaresab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.
Protocols
References
This product has been referenced in:
- Bian F et al. Polycomb repressive complex 1 controls uterine decidualization. Sci Rep 6:26061 (2016). Read more (PubMed: 27181215) »
- Jacinto FV et al. The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev 29:1224-38 (2015). Read more (PubMed: 26080816) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"