Overview

  • Product name
    Anti-RYBP antibody [EPR13059(2)]
    See all RYBP primary antibodies
  • Description
    Rabbit monoclonal [EPR13059(2)] to RYBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, Flow Cyt, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RYBP aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N488

  • Positive control
    • K562, SW480, HepG2 and rat PC12 cell lysates; Human placenta and Human clear carcinoma of kidney tissues; HepG2, SW480 and K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
  • Tissue specificity
    Widely expressed with highest levels in lymphoid tissues and placenta.
  • Sequence similarities
    Contains 1 RanBP2-type zinc finger.
  • Cellular localization
    Nucleus. Cytoplasm. Primarily found in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410018J24Rik antibody
    • AAP 1 antibody
    • AAP1 antibody
    • APAP 1 antibody
    • APAP-1 antibody
    • APAP1 antibody
    • Apoptin associating protein 1 antibody
    • Apoptin-associating protein 1 antibody
    • cb337 antibody
    • Death effector domain associated factor antibody
    • Death effector domain-associated factor antibody
    • Death effector domain-associated factor B antibody
    • DED associated factor antibody
    • DED-associated factor antibody
    • DED-associated factor B antibody
    • DEDAF antibody
    • MGC62213 antibody
    • RING1 and YY1 binding protein a antibody
    • RING1 and YY1 binding protein antibody
    • RING1 and YY1-binding protein antibody
    • RING1 and YY1-binding protein B antibody
    • Ring1 interactor RYBP antibody
    • RYBP antibody
    • RYBP_HUMAN antibody
    • rybpa antibody
    • wu:fi34f03 antibody
    • YEAF 1 antibody
    • YEAF1 antibody
    • YY1 and E4TF1 associated factor 1 antibody
    • YY1 and E4TF1-associated factor 1 antibody
    • YY1- and E4TF1/GABP-associated factor 1 antibody
    • zgc:153842 antibody
    see all

Images

  • All lanes : Anti-RYBP antibody [EPR13059(2)] (ab185971) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • Anti-RYBP antibody [EPR13059(2)] (ab185971) at 1/10000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?

  • Anti-RYBP antibody [EPR13059(2)] (ab185971) at 1/1000 dilution + rat PC12 cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 25 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded, Human placenta tissue labeling RYBP with ab185971 at a 1/100 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded, Human clear cell carcinoma kidney tissue labeling RYBP with ab185971 at a 1/100 dilution. Counter stained with hematoxylin.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling RYBP with ab185971 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, SW480 cells labeling RYBP with ab185971 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

  • Flow cytometry analysis of paraformaldehyde-fixed K562 cells labeling RYBP with ab185971 at a 1/40 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling RYBP with ab185971 at 1/50 dilution and goat anti-rabbit IgG, (H+L), peroxidase conjugated at a 1/1000 dilution.

References

ab185971 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (forelimb)
Permeabilization
No
Specification
forelimb
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

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Verified customer

Submitted May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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